| 基于拉曼光谱和多元统计分析研究血清标本及在冠心病诊断中的应用 |
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| 引用本文： | 印晓静,胡嘉波,韩崇旭,冯秀丽,府伟灵,张阳. 基于拉曼光谱和多元统计分析研究血清标本及在冠心病诊断中的应用[J]. 中华全科医学, 2019, 17(10): 1621. DOI: 10.16766/j.cnki.issn.1674-4152.001012 |
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| 作者姓名： | 印晓静 胡嘉波 韩崇旭 冯秀丽 府伟灵 张阳 |
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| 作者单位： | 1. 江苏大学医学院检验医学系, 江苏 镇江 212013; |
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| 基金项目： | 国家重点基础研究发展计划973项目(2015CB755400)；国家自然科学基金重点项目(81430054) |
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| 摘 要： | 目的 探讨显微拉曼共焦系统在冠心病(coronary heart disease,CHD)患者血清诊断中的应用价值。 方法 选取2018年8月—2019年1月在陆军军医大学第一附属医院收集的96份血清样本，包括46例经冠脉造影明确诊断的冠心病组血清和50例体检健康组血清，运用XploRA PLUS全自动显微共焦拉曼系统，测量并分析各标本的血清拉曼光谱特征，并联合多元统计中的正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis，OPLS-DA)等，使用SIMCA-P 14.1分析软件进行统计学处理。 结果 冠心病组和健康组的平均拉曼谱峰形态相似；在位移1 080.47/cm、1 102.01/cm、1 127.52/cm、1 335.46/cm、1 603.83/cm、1 657.69/cm处，冠心病组的振动强度高于健康组；在位移1 154.51/cm、1 516.81/cm处，健康组的振动强度显著高于冠心病组，这些谱峰的变化可能有利于血清拉曼光谱对冠心病的筛查；OPLS-DA模型的R2X(cum)=0.818，R2Y(cum)=0.644，模型的可预测性Q2(cum)=0.497，基于OPLS-DA冠心病组和健康组的AUC分别为0.970和0.970。 结论 显微共焦拉曼系统联合OPLS-DA分析，可对已知冠心病组和健康组血清样本进行快速便捷的区分，作为探索性实验方法，为冠心病的检测诊断提供了一种新的可能。
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| 关 键 词： | 血清拉曼光谱 多元统计分析 冠心病 鉴别诊断 |
| 收稿时间： | 2019-02-12 |
Application of Raman spectroscopy and multivariate statistical analysis in serum diagnosis of coronary heart disease |
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| Affiliation: | Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China |
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| Abstract: | Objective To discuss the application of micro-Raman confocal system in the test of serum sample of coronary heart disease (CHD). Methods The serum samples of 46 patients with coronary heart disease confirmed by coronary angiography and 50 subjects receiving healthy physical examination were selected in the First Affiliated Hospital of Army Medical University between August 2018 and January 2019. The XploRA PLUS automatic microscopic confocal Raman system was used to measure and analyze the Raman spectrum of serum. Statistical processing was performed by orthogonal partial least squares discriminant analysis (OPLS-DA) by using SIMCA-P14.1 analysis software. Results The Raman spectrum mean peak of the two groups was similar; At the displacement of 1 080.47/cm, 1 102.01/cm, 1 127.52/cm, 1 335.46/cm, 1 603.83/cm, 1 657.69/cm, the vibration intensity of the CHD group was higher than that of the control group, At the displacement of 1 154.51/cm, 1 516.81/cm, the vibration intensity results were just the opposite. The changes of these spectral peaks may be beneficial to the screening of CHD by Raman spectra; R2X (cum) of the OPLS-DA model was 0.881, R2Y (Cum) was 0.644, the predictability of the model Q2 (cum) was 0.497, and the AUC based on OPLS-DA were 0.970 and 0.970, respectively. Conclusion The microscopic confocal Raman system combined with OPLS-DA analysis can quickly and conveniently distinguish the serum samples from the known coronary heart disease group and the healthy group, providing a new possibility for the detection and diagnosis of coronary heart disease as an exploratory experimental method. |
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