| 藏红花素对大鼠视网膜缺血-再灌注损伤的保护作用 |
| |
| 引用本文： | 常花蕾,朱娟,于敬妮,田蕴霖. 藏红花素对大鼠视网膜缺血-再灌注损伤的保护作用[J]. 眼科新进展, 2019, 0(7): 630-634. DOI: 10.13389/j.cnki.rao.2019.0145 |
| |
| 作者姓名： | 常花蕾 朱娟 于敬妮 田蕴霖 |
| |
| 作者单位： | 710004 陕西省西安市，陕西省眼科中心、西安市第四医院眼科、西安交通大学医学院附属广仁医院眼科 |
| |
| 摘 要： | 目的观察大鼠视网膜缺血-再灌注损伤(retinal ischemia-reperfusion injury,RIRI)时藏红花素对视网膜细胞凋亡数目、凋亡相关蛋白Caspase-3表达的影响,探讨缺血再灌注时藏红花素对视网膜的保护作用机制。方法选取体质量200~250 g的健康雄性SD大鼠24只,随机分为4组:对照组、模型组、藏红花素低剂量组和藏红花素高剂量组,每组6只。藏红花素低剂量组、高剂量组分别于造模前3 d、30 min定时腹腔注射5 g·L-1藏红花素5 mg·kg-1、50 mg·kg-1。造模成功后24 h处死大鼠并摘取眼球。使用电镜观察各组大鼠视网膜细胞结构,TUNEL染色检测视网膜凋亡细胞数量,免疫组织化学染色法观察凋亡相关蛋白Caspase-3在视网膜中的表达。结果视网膜TUNEL染色发现,对照组视网膜组织中几乎未发现凋亡细胞的阳性表达,模型组视网膜神经节细胞层和内核层中发现大量棕黄色着色的细胞,其凋亡细胞数为(27.40±0.96)个,藏红花素低剂量组可见神经节细胞层、内核层凋亡细胞数较模型组减少,凋亡细胞数为(8.40±0.41)个,与模型组相比差异有统计学意义(P<0.01);藏红花素高剂量组凋亡细胞数较模型组和低剂量组明显减少,凋亡细胞数为(4.30±0.47)个,和藏红花素低剂量组相比差异有统计学意义(P<0.01)。对照组大鼠视网膜组织Caspase-3染色阴性,模型组视网膜神经节细胞层可见棕黄色颗粒位于细胞浆内,藏红花素低剂量组Caspase-3蛋白表达量与模型组类似,藏红花素高剂量组Caspase-3蛋白表达量与对照组类似。结论藏红花素能通过降低Caspase-3蛋白含量及凋亡细胞数,从而有效保护视网膜免受RIRI。
|
| 关 键 词： | 藏红花素 凋亡 视网膜缺血-再灌注损伤 CASPASE-3 |
Protective effect of crocin against retinal ischemia-reperfusion injury in rats |
| |
| CHANG Hua-Lei,ZHU Juan,YU Jing-Ni,TIAN Yun-Lin. Protective effect of crocin against retinal ischemia-reperfusion injury in rats[J]. Recent Advances in Ophthalmology, 2019, 0(7): 630-634. DOI: 10.13389/j.cnki.rao.2019.0145 |
| |
| Authors: | CHANG Hua-Lei ZHU Juan YU Jing-Ni TIAN Yun-Lin |
| |
| Affiliation: | Shaanxi Ophthalmic Medical Center，Department of Ophthalmology，Xi’an Fourth Hospital，Affiliated Guangren Hospital，School of Medicine，Xi’an Jiaotong University，Xi’an 710004，Shaanxi Province，China |
| |
| Abstract: | Objective To observe the effect of crocin on the number of apoptotic cells and apoptosis-related protein in rat retina after retinal ischemia-reperfusion injury(RIRI)in rats， and to investigate the protective mechanism of crocin on retina during ischemia reperfusion.Methods Twenty four male Sprague-Dawley (SD) rats (200-250 g) were used in this study.All experiments were randomly divided into 4 groups:normal group (n=6),ischemia reperfusion injury group (n=6),crocin-treated low dose group (n=6) and high dose group (n=6).Rats were pre-treated in low dose group (5 mg·kg-1) and high dose group(50 mg·kg-1) with crocin (5 g·L-1) via intraperitoneal injection 30 minutes and once daily three days before retinal IR injury.After successful modeling,the rats were executed and their eyeballs were picked.Electron microscopy was used to examine the structural alteration in retinal cells.Apoptosis of retinal cells were detected using the TUNEL technique.Expression levels of Caspase-3 were investigated by immunohistochemistry.Results Measurement of apoptosis by TUNEL technique revealed that there was hardly positive apoptosis in the retinal tissue of normal group,while ischemia reperfusion injury group showed a large number of apoptosis with brown-yellow in the inner nuclear and ganglion cell layer.And the number of apoptotic cells was about (27.40±0.96).The number of apoptotic cells of crocin-treated low dose group in the inner nuclear and ganglion cell layer was decreased compared with that of the model group,and the number of apoptotic cells was (8.40±0.41),which was statistically different from that of the model group (P<0.01).The number of apoptotic cells of the high dose group of crocin was significantly lower than that in the model group and low dose group,and the number of apoptotic cells was about (4.30±0.47).The difference is statistically significant (P<0.01) compared with low dose group.The pattern of Caspase-3 protein expression indicated negative of retinal tissue in the normal group.The model group showed that the brown-yellow particles were located in the cytoplasm in the cell layer,and the expression of Caspase-3 protein in the low dose group was similar to that of the model group.The expression of Caspase-3 protein in high dose group was similar to that in normal group.Conclusion Crocin can effectively protect the retina from ischemia-reperfusion injury by reducing the content of Caspase-3 protein and the number of apoptotic cells. |
| |
| Keywords: | crocin apoptosis ischemia-reperfusion Caspase-3 |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《眼科新进展》浏览原始摘要信息 |
|
点击此处可从《眼科新进展》下载免费的PDF全文 |
|
