Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1 |
| |
| Authors: | Chijiang Gu Mingyuan Zhang Weiliang Sun Changzheng Dong |
| |
| Affiliation: | * Department of Gastrointestinal Surgery, Affiliated Yinzhou Hospital of Ningbo University, Ningbo City, Zhejiang Province, P.R. China† School of Medicine, Ningbo University, Ningbo City, Zhejiang Province, P.R. China |
| |
| Abstract: | Colorectal cancer (CRC) is a common clinical cancer that remains incurable in most cases. miRNAs are reported to play a part in the development of various tumors. In the present study, we found that miR-324-5p was downregulated in CRC cells, while ELAV (embryonic lethal, abnormal vision, Drosophila)-like protein 1 (ELAVL1) showed a higher expression. miR-324-5p transfection significantly inhibited the proliferation as well as invasion in both SW620 and SW480 cells. miR-324-5p mimic transfection markedly decreased the expression of ELAVL1. Luciferase reporter gene assay confirmed that ELAVL1 is a direct target of miR-324-5p. Furthermore, cancer invasion factors uPA, uPAR, and MMP-9 were found to drop significantly in miR-324-5p-transfected groups. To conclude, our findings indicate that miR-324-5p may play a suppressive role in colorectal cell viability and invasion, at least in part, through directly targeting ELAVL1. Therefore, miR-234-5p might function as a promising candidate for CRC treatment and deserves deeper research. |
| |
| Keywords: | Colorectal cancer (CRC) miR-324-5p Proliferation Invasion Drosophila)-like protein 1 (ELAVL1)" target=" _blank" >(Embryonic lethal, abnormal vision, Drosophila)-like protein 1 (ELAVL1) |
|
| 点击此处可从《Oncology research》浏览原始摘要信息 |
|
点击此处可从《Oncology research》下载全文 |
|
