| lncRNA Xist/miR-215-5p下调LPAR4表达对乳腺癌细胞增殖与转移的影响 |
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| 引用本文： | 袁甫军1,张 帆2,全卫涛3,金依笑4,王健生4. lncRNA Xist/miR-215-5p下调LPAR4表达对乳腺癌细胞增殖与转移的影响[J]. 现代肿瘤医学, 2019, 0(15): 2656-2661. DOI: 10.3969/j.issn.1672-4992.2019.15.009 |
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| 作者姓名： | 袁甫军1 张 帆2 全卫涛3 金依笑4 王健生4 |
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| 作者单位： | 1.武功县人民医院普通外科，陕西 咸阳 712200;2.神木市医院肿瘤外科，陕西 神木 719300;3.商洛市中医医院普通外科，陕西 商洛 726000;4.西安交通大学第一附属医院肿瘤外科，陕西 西安 710061 |
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| 基金项目： | National Natural Science Foundation of China(No.81502295）；国家自然科学基金项目（编号:81502295） |
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| 摘 要： | 目的:探讨溶血磷脂酸受体4（LPAR4)在乳腺癌组织中的表达水平及与乳腺癌发生发展的关系，探究lncRNA Xist/miR-215-5p对LPAR4的调控作用。方法:通过Targetscan和Starbase预测LPAR4的上游通路lncRNA Xist/miR-215-5p。使用慢病毒转染LPAR4和miR-215-5p；使用质粒转染lncRNA Xist。在MDA-MB-231细胞系中，通过MTT和划痕实验检测细胞的增殖和转移能力。结果:LPAR4在乳腺癌细胞系中高表达，miR-215-5p和lncRNA Xist为低表达。Western blot显示LPAR4在乳腺癌细胞系中高表达。MTT和划痕实验提示上调LPAR4可以促进MDA-MB-231细胞的增殖和转移能力。进一步实验发现lncRNA Xist可以上调miR-215-5p，并且负性调控LPAR4；且lncRNA Xist可以抑制MDA-MB-231细胞的增殖能力。除此之外，在lncRNA Xist下调细胞中，上调miR-215-5p可以抑制lncRNA Xist下调介导的细胞增殖能力增强。结论:lncRNA Xist/miR-215-5p可能通过调控LPAR4的表达，为治疗乳腺癌提供可能的新靶点。LPAR4在乳腺癌细胞中高表达，可能成为乳腺癌潜在的肿瘤标志物。
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| 关 键 词： | LPAR4 lncRNA Xist miR-215-5p 预后 增殖 转移 |
Downregulation of lncRNA Xist/miR-215-5p regulates LPAR4 to inhibit proliferation and migration of breast cancer cells |
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| Yuan Fujun1,Zhang Fan2,Quan Weitao3,Jin Yixiao4,Wang Jiansheng4. Downregulation of lncRNA Xist/miR-215-5p regulates LPAR4 to inhibit proliferation and migration of breast cancer cells[J]. Journal of Modern Oncology, 2019, 0(15): 2656-2661. DOI: 10.3969/j.issn.1672-4992.2019.15.009 |
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| Authors: | Yuan Fujun1 Zhang Fan2 Quan Weitao3 Jin Yixiao4 Wang Jiansheng4 |
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| Affiliation: | 1.Department of General Surgery，Wugong County People's Hospital，Shaanxi Xianyang 712200，China；2.Department of Surgical Oncology，Shenmu Hospital，Shaanxi Shenmu 719300，China；3.Department of General Surgery，Shangluo Traditional Chinese Medicine Hospital，Shaanxi Shangluo 726000，China；4.Department of Surgical Oncology，First Affiliated Hospital of Xi'an Jiaotong University，Shaanxi Xi'an 710061，China. |
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| Abstract: | Objective:To evaluate the expression of LPAR4 in breast cancer tissues and its association with breast cancer progression，and the regulating role of lncRNA Xist/miR-215-5p for LPAR4.Methods:The expression of LPAR4，lncRNA Xist and miR-215-5p in breast cancer cell line was tested by qRT-PCR.LPAR4 and miR-215-5p lentivirus and Xist plasmid were transfected.In MDA-MB-231 cell line，MTT and wound healing tests were used to investigate the proliferation and migration ability.Results:qRT-PCR indicated that LPAR4 expressed higher in breast cancer cell line，and miR-215-5p and Xist were expressed lower.MTT and wound healing test indicated that up regulation of LPAR4 could promote the proliferation and migration of MDA-MB-231 cell line.Furthermore，Xist could up regulate miR-215-5p and down regulate LPAR4，and Xist could inhibit the proliferation of MDA-MB-231.In addition，in Xist down regulated cell，up regulation of miR-215-5p could inhibit the Xist-associated enhanced cell proliferation.Conclusion:LPAR4 was associated with poorer prognosis in breast cancer and had the potential to be a novel biomarker.lncRNA Xist/miR-215-5p could provide novel therapeutic targets for breast cancer by targeting LPAR4. |
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| Keywords: | LPAR4 lncRNA Xist miR-215-5p prognosis proliferation migration |
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