| 高效液相色谱法测定大鼠血浆中的西他列汀浓度及其药动学研究(英文) |
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| 引用本文： | 酒向飞,尚德为,陈烨,李新刚,万小蒙,周田彦,卢炜. 高效液相色谱法测定大鼠血浆中的西他列汀浓度及其药动学研究(英文)[J]. 中国药学, 2011, 20(1): 63. DOI: 10.5246/jcps.2011.01.009 |
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| 作者姓名： | 酒向飞 尚德为 陈烨 李新刚 万小蒙 周田彦 卢炜 |
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| 作者单位： | 北京大学医学部药学院药剂学系;北京大学医学部天然药物及仿生药物国家重点实验室; |
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| 基金项目： | National Integrity Innovational Technology Platform of New Drug and Research and Development (Grant No. 2009ZX09301-010); National Fund for Talent Training in Basic Science |
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| 摘 要： | 本研究建立并验证了测定大鼠血浆中磷酸西他列汀浓度的高效液相色谱法,并在大鼠体内进行药动学研究。血浆中的药物和内标(氢化可的松)由乙酸乙酯萃取。所用色谱柱为Agilent Zorbax Extend-C18柱(250mm×4.6mm,4μm),进样量为20μL,检测波长为267nm,柱温为30oC。流动相为甲醇–水(60:40,内含10mMTris和10mM三乙胺,并用1M盐酸调至pH9.0)。流速为1.0mL/min。色谱峰具有良好的特异性。在0.75–100.0μg/mL范围内具有良好的线性关系(r>0.9957)。定量下限为0.75μg/mL。日间,日内精密度均小于10%。在0.75,10.0,100.0μg/mL的相对回收率为105.3%,99.8%,99.0%,提取回收率分别为81.5%,82.4%,84.5%。样品的稳定性良好。运用该方法测定了大鼠口服磷酸西他列汀水溶液后的血药浓度,说明本方法可用于药物动力学的研究。
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| 关 键 词： | 西他列汀 高效液相色谱 大鼠血浆 药物动力学 |
A high performance liquid chromatography method for the quantitative determination assay of sitagliptin in rat plasma and its application in pharmacokinetics study |
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| Xiang-Fei Jiu ,De-Wei Shang ,Ye Chen ,Xin-Gang Li ,Xiao-Meng Wan ,Tian-Yan Zhou ,,Wei Lu , . State Key Laboratory of Natural , Biomimetic Drugs,Peking University Health Science Center,Beijing ,China. A high performance liquid chromatography method for the quantitative determination assay of sitagliptin in rat plasma and its application in pharmacokinetics study[J]. Journal of Chinese Pharmaceutical Sciences, 2011, 20(1): 63. DOI: 10.5246/jcps.2011.01.009 |
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| Authors: | Xiang-Fei Jiu De-Wei Shang Ye Chen Xin-Gang Li Xiao-Meng Wan Tian-Yan Zhou Wei Lu . State Key Laboratory of Natural Biomimetic Drugs Peking University Health Science Center Beijing China |
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| Affiliation: | Xiang-Fei Jiu 2,De-Wei Shang 2,Ye Chen 2,Xin-Gang Li 2,Xiao-Meng Wan 2,Tian-Yan Zhou 1,2*,Wei Lu 1,2* 1. State Key Laboratory of Natural and Biomimetic Drugs,Peking University Health Science Center,Beijing 100191,China 2. Department of Pharmaceutics,School of Pharmaceutical Sciences,China |
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| Abstract: | |
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| Keywords: | Sitagliptin HPLC Rat plasma Pharmacokinetics |
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