| 二甲双胍改善佐剂性关节炎大鼠骨破坏的作用和机制研究 |
| |
| 引用本文： | 陈笑天,宋宜宁,王颖,周静,王茜,司悦,魏芳. 二甲双胍改善佐剂性关节炎大鼠骨破坏的作用和机制研究[J]. 蚌埠医学院学报, 2021, 46(6): 701-707. DOI: 10.13898/j.cnki.issn.1000-2200.2021.06.001 |
| |
| 作者姓名： | 陈笑天 宋宜宁 王颖 周静 王茜 司悦 魏芳 |
| |
| 作者单位： | 1.蚌埠医学院第一附属医院 骨科, 安徽 蚌埠 2330042.蚌埠医学院 药学院, 安徽 蚌埠 233030 |
| |
| 基金项目： | 国家自然科学基金项目81703529国家级大学生创新训练项目201810367049蚌埠医学院自然科学基金面上项目BYKY18122 |
| |
| 摘 要： | 目的:探讨二甲双胍治疗类风湿性关节炎(rheumatoidrthritis arthritis,RA)骨破坏的作用及机制.方法:采用Freund完全佐剂诱导佐剂性关节炎(adjuvant arthritis,AA)大鼠模型.全身评分评价不同剂量二甲双胍对AA大鼠炎症的作用;对各组大鼠踝关节和足部通过X射线成像进行检查;...
|
| 关 键 词： | 类风湿性关节炎 骨破坏 二甲双胍 大鼠 |
| 收稿时间： | 2020-09-02 |
Study on the effects and mechanism of metformin on bone destruction induced by adjuvant arthritis in rats |
| |
| CHEN Xiao-tian,SONG Yi-ning,WANG Ying,ZHOU Jing,WANG Qian,SI Yue,WEI Fang. Study on the effects and mechanism of metformin on bone destruction induced by adjuvant arthritis in rats[J]. Journal of Bengbu Medical College, 2021, 46(6): 701-707. DOI: 10.13898/j.cnki.issn.1000-2200.2021.06.001 |
| |
| Authors: | CHEN Xiao-tian SONG Yi-ning WANG Ying ZHOU Jing WANG Qian SI Yue WEI Fang |
| |
| Affiliation: | 1.Department of Orthopaedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 2330042.School of Pharmacy, Bengbu Medical College, Bengbu Anhui 233030, China |
| |
| Abstract: | ObjectiveTo investigate the effects and mechanism of metformin on bone destruction in rheumatoid arthritis(RA).MethodsThe adjuvant arthritis(AA) models with complete Freund's adjuvant(CFA) were established in rats.The systemic inflammation score was used to evaluate the effects of different doses of metformin on inflammation in AA rats; the ankle and foot of each group were examined using X-ray imaging; ELISA was used to measure the serum calcium, phosphorus, osteoprotectin(OPG), nuclear factor κB receptor activator ligand(RANKL) in peripheral blood of rats; the expression levels of AMP-activated protein kinase(AMPK), interleukin-6(IL-6) and tumor necrosis factor α(TNF-α) were measured by immunohistochemistry.The effects of metformin on bone destruction in rats was evaluated.ResultsMetformin could improve the systemic inflammatory response, and reduce paw swelling in AA rats, especially at high dose.All dose metformin groups could significantly improve the local bone mineral density and bone destruction in AA rats.The serum levels of calcium and phosphorus ion increased in high-dose metformin group.Different doses of metformin could increase the serum OPG level, and decrease the serum RANKL level.Compared with the model group, metformin could increase the protein expression of AMPK, decrease the protein expression of IL-6 and TNF-α, and the differences of which were statistically significant(P<0.05 to P<0.01).ConclusionsMetformin plays a protective role on the bone destruction of RA.The mechanism of action may be related to the effects of anti-inflammation, regulation of serum bone metabolism index and activation of AMPK signaling pathway to affect the OPG-RANKL-RANK system. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《蚌埠医学院学报》浏览原始摘要信息 |
|
点击此处可从《蚌埠医学院学报》下载免费的PDF全文 |
|
