| 胃癌组织中RASGRF1基因甲基化及蛋白表达与其临床意义 |
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| 引用本文： | 唐维平,林珺珺,李启信. 胃癌组织中RASGRF1基因甲基化及蛋白表达与其临床意义[J]. 蚌埠医学院学报, 2021, 46(9): 1223-1226. DOI: 10.13898/j.cnki.issn.1000-2200.2021.09.018 |
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| 作者姓名： | 唐维平 林珺珺 李启信 |
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| 作者单位： | 安徽医科大学附属巢湖医院 肠胃外科, 安徽 巢湖 238000 |
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| 摘 要： | 目的:探讨胃癌组织中Ras蛋白特异性鸟苷酸释放因子1(RASGRF1)基因甲基化及蛋白表达以及临床意义.方法:收集80例胃癌病人病历资料,采用免疫组织化学法检测80例胃癌组织和相应癌旁组织中RASGRF1蛋白表达情况,采用甲基化特异性PCR检测RASGRF1基因甲基化状态,分析胃癌组织中RASGRF1基因甲基化及蛋白表...
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| 关 键 词： | 胃肿瘤 Ras蛋白特异性鸟苷酸释放因子1 基因甲基化 |
| 收稿时间： | 2020-11-27 |
Methylation and protein expression of RASGRF1 gene in gastric cancer and its clinical significance |
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| TANG Wei-ping,LIN Jun-jun,LI Qi-xin. Methylation and protein expression of RASGRF1 gene in gastric cancer and its clinical significance[J]. Journal of Bengbu Medical College, 2021, 46(9): 1223-1226. DOI: 10.13898/j.cnki.issn.1000-2200.2021.09.018 |
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| Authors: | TANG Wei-ping LIN Jun-jun LI Qi-xin |
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| Affiliation: | Department of Gastroenterology, Chaohu Hospital, Anhui Medical University, Chaohu Anhui 238000, China |
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| Abstract: | ObjectiveTo investigate the methylation and protein expression of Ras specific guanylate releasing factor 1(RASGRF1) in gastric cancer and its clinical significance.MethodsThe clinical data of 80 patients with gastric cancer were collected.The expression levels of RASGRF1 protein in 80 gastric cancer tissues and corresponding adjacent tissues were detected using immunohistochemistry.The methylation status of RASGRF1 gene was detected by methylation specific PCR.Spearman correlation was used to analyze the correlation of methylation with protein expression levels of RASGRF1 in gastric cancer tissues.The relationship between the methylation status and protein expression levels were observed.ResultsThe RASGRF1 protein expression and gene methylation expression were different between gastric cancer tissues and adjacent tissues(P < 0.05).The results of Spearman correlation analysis showed that the RASGRF1 protein expression was negatively correlated with the gene methylation in gastric cancer tissues(P < 0.01).The RASGRF1 protein expression in gastric cancer tissues was not related to the gender, age, tumor diameter, histological differentiation degree and distant metastasis(P>0.05), but was related to tumor stage(P < 0.05).The RASGRF1 gene methylation in gastric cancer tissues was not related to the gender, age, tumor diameter and histological differentiation(P>0.05), but was related to the tumor stage and distant metastasis(P < 0.05 and P < 0.01).ConclusionsThere is a negative correlation between RASGRF1 gene methylation and protein expression in gastric cancer tissues.The RASGRF1 gene methylation and protein expression have a certain correlation with tumor stage and distant metastasis. |
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