| 铁过载对性成熟期雄性大鼠生育能力的影响 |
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| 引用本文： | 张华琦,马爱国,孙永叶,钟凤,蔡静,何丽敏. 铁过载对性成熟期雄性大鼠生育能力的影响[J]. 癌变．畸变．突变, 2017, 29(2): 134-138,142. DOI: 10.3969/j.issn.1004-616x.2017.02.012 |
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| 作者姓名： | 张华琦 马爱国 孙永叶 钟凤 蔡静 何丽敏 |
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| 作者单位： | 青岛大学公共卫生学院营养与食品卫生学教研室,山东 青岛,266021;青岛大学公共卫生学院营养与食品卫生学教研室,山东 青岛,266021;青岛大学公共卫生学院营养与食品卫生学教研室,山东 青岛,266021;青岛大学公共卫生学院营养与食品卫生学教研室,山东 青岛,266021;青岛大学公共卫生学院营养与食品卫生学教研室,山东 青岛,266021;青岛大学公共卫生学院营养与食品卫生学教研室,山东 青岛,266021 |
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| 基金项目： | 中国博士后科学基金面上资助项目，青岛市博士后应用研究项目，青岛大学医学院创新团队青年教师培育项目 |
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| 摘 要： | 目的：研究铁过载对性成熟期雄性大鼠生育能力的影响。方法：6~7周龄SPF级雄性Wistar大鼠40只，按体质量随机分为对照组、缺铁组、低剂量铁过载组及高剂量铁过载组，每组10只，经腹腔注射含Fe2+分别为0.9、0.3、9和18 mg的右旋糖酐铁注射液，隔日注射1次，连续6周。于第6周末经腹主动脉采血，采用比色法检测血清铁含量。留取睾丸及附睾，计算脏器指数，制备精子悬液，显微镜下观察精子数量、精子运动情况及精子畸形情况。采用可见光法检测睾丸组织中过氧化氢酶（CAT）水平，黄嘌呤氧化酶法检测睾丸组织中总超氧化物歧化酶（T-SOD）水平，比色法检测睾丸组织中铁含量及谷胱甘肽过氧化物酶（GSH-Px）水平，硫代巴比妥酸法检测睾丸组织中丙二醛（MDA）水平。结果：缺铁组大鼠血清铁含量明显低于对照组（P < 0.01），而睾丸组织中铁含量与对照组比较，差异无统计学意义（P > 0.05）。低剂量和高剂量铁过载组大鼠血清和睾丸组织中的铁含量均显著高于对照组（P < 0.01）。与对照组相比，缺铁组大鼠的精子数量、精子活动率及精子畸形率的差异均无统计学意义（P > 0.05）。低剂量铁过载组大鼠的精子活动率显著低于对照组（P < 0.01）。高剂量铁过载组大鼠的精子数量和精子活动率显著低于对照组和低剂量铁过载组，且精子畸形率显著高于对照组和低剂量铁过载组（P < 0.05）。缺铁组大鼠睾丸GSH-Px活性显著低于对照组（P < 0.05）。低剂量铁过载组大鼠睾丸GSH-Px活性显著低于对照组，而MDA水平显著高于对照组（P < 0.05）。高剂量铁过载组大鼠睾丸CAT水平及GSH-Px活性均显著低于对照组和低剂量铁过载组，而MDA水平显著高于对照组和低剂量铁过载组（P < 0.05）。结论：铁过载可影响性成熟期雄性大鼠的生育能力，表现为精子数量减少、精子运动率降低以及精子畸形率升高，其原因可能与铁过载所导致的高氧化应激水平有关。
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| 关 键 词： | 铁过载 精子数量 精子活动率 精子畸形率 氧化应激 |
| 收稿时间： | 2016-08-24 |
Effect of iron overload on fertility of sexually mature male rats |
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| ZHANG Huaqi,MA Aiguo,SUN Yongye,ZHONG Feng,CAI Jing,HE Limin. Effect of iron overload on fertility of sexually mature male rats[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2017, 29(2): 134-138,142. DOI: 10.3969/j.issn.1004-616x.2017.02.012 |
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| Authors: | ZHANG Huaqi MA Aiguo SUN Yongye ZHONG Feng CAI Jing HE Limin |
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| Affiliation: | Department of Nutrition and Food Hygiene, Public Health College, Qingdao University, Qingdao 266021, Shandong, China |
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| Abstract: | OBJECTIVE:The aim of the study was to observe the effect of iron overload on fertility of sexually mature male rats.METHODS:According to bodyweights,40 healthy male Wistar rats were randomly divided into four groups:control,iron deficiency,low-dose iron overloadand high-dose iron overload groups. All rats were injected 2+intraperitoneally every other day. The content of the Fe in these four groups were 0.9,0.3,9 and 18 mg,respectively. After 6 weeks,blood samples from these rats were collected to determine serum iron levels. Testes and epididymides were removed and weighed. Sperm numbers were counted. Sperm mobility and teratosperm rates were calculated. Iron concentration,catalase(CAT),totalsuperoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)and malonaldehyde (MDA) levels in the testes were determined.RESULTS:Compared with the control group,the serum iron concentration of the iron deficiency group was significantly lower (P<0.01). However,there was no significant difference in the iron concentrations of the testes (P>0.05). The serum and testes iron concentrations in the low- and high-doses iron overload groups were both significantly higher than that in the control group (all P<0.01). There was no significant difference in the sperm count,the sperm motility rate and the teratosperm rate between the iron deficiency group and the control group (all P>0.05). Compared with the control group,the sperm motility rate in the low-dose iron overload group was significantly decreased (P<0.01);the sperm count and the sperm motility rate in the high-dose iron overload group were all significantly lower than those of control group and low-dose iron overload group,but the teratosperm rate was significantly higher than that of control group and low-dose iron overload group (allP<0.05). The rats in the iron deficiency group had significantly lower GSH-Px l evels in the testis comparing with the control group. Moreover,compared with the control group,the GSH-Px levels in the testis of the low-dose iron overload group were significantly lower,but the MDA level was significantly higher (all P<0.05). The CAT and GSH-Px levels in the testis of the high-dose iron overload group were all significantly lower than those of control group and low-dose iron overload group,but the MDA level was significantly higher than that of control group and low-dose iron overload group (all P<0.05).CONCLUSION:Iron overload can affect the fertility of sexually mature male rats,including reduced sperm count,decreased sperm motility rate and increased teratosperm rate. These adverse effects may be related to the increased oxidatives tress level caused b y on overload. |
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| Keywords: | iron overload sperm count sperm motility rate teratosperm rate oxidative stress |
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