Epidermal growth factor receptor (EGFR)—MAPK—nuclear factor(NF)‐κB—IL8: A possible mechanism of particulate matter(PM) 2.5‐induced lung toxicity |
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| Authors: | Seung‐Chan Jeong Yoon Cho Mi‐Kyung Song Eunil Lee Jae‐Chun Ryu |
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| Affiliation: | 1. Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology, Seoul, Korea;2. Department of Preventive Medicine, Korea University, Korea Project for Reducing Light Pollution Effects to Human and Ecosystem, Korea;3. National Center for Efficacy evaluation for Respiratory disease product, Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup, Jeollabuk‐do, Republic of Korea;4. Department of Pharmacology and Toxicology, Human and Environmental Toxicology, Korea University of Science and Technology, Daejeon, Korea |
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| Abstract: | Airway inflammation plays a central role in the pathophysiology of diverse pulmonary diseases. In this study, we investigated whether exposure to particulate matter (PM) 2.5, a PM with an aerodynamic diameter of less than 2.5 µm, enhances inflammation‐related toxicity in the human respiratory system through activation of the epidermal growth factor receptor (EGFR) signaling pathway. Through cytokine antibody array analysis of two extracts of PM2.5 [water (W‐PM2.5) and organic (O‐PM2.5) soluble extracts] exposed to A549 (human alveolar epithelial cell), we identified eight cytokines changed their expression with W‐PM2.5 and three cytokines with O‐PM2.5. Among them, epidermal growth factor (EGF) was commonly up‐regulated by W‐PM2.5 and O‐PM2.5. Then, in both groups, we can identify the increase in EGF receptor protein levels. Likewise, increases in the phosphorylation of ERK1/2 MAP kinase and acetylation of nuclear factor(NF)‐κB were detected. We also detected an increase in IL‐8 that was related to inflammatory response. And using the erlotinib as an inhibitor of EGFR, we identified the erlotinib impaired the phosphorylation of EGFR, ERK1/2, acetylation of NF‐κB proteins and decreased IL‐8. Furthermore, at in vivo model, we were able to identify similar patterns. These results suggest that PM2.5 may contribute to an abnormality in the human respiratory system through EGFR, MAP kinase, NF‐κB, and IL‐8 induced toxicity signaling. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1628–1636, 2017. |
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| Keywords: | Cytokine epidermal growth factor receptor (EGFR) erlotinib mitogen‐activated protein kinase (MAPK) particulate matter2.5(PM2.5) |
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