| 大肠埃希菌(EHEC/EPEC)紧密黏附素基因的分型分析 |
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| 引用本文： | 陈强,卢珊,赵爱兰,熊衍文. 大肠埃希菌(EHEC/EPEC)紧密黏附素基因的分型分析[J]. 疾病监测, 2012, 27(2): 93-96 |
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| 作者姓名： | 陈强 卢珊 赵爱兰 熊衍文 |
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| 作者单位： | 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京,102206 |
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| 基金项目： | 国家“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(No.2008ZX10004-009)~~ |
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| 摘 要： | 目的 了解大肠埃希菌分离株eaeA基因的多态性。 方法 对不同来源的60株大肠埃希菌分离株的eaeA基因进行聚合酶链反应扩增后直接测序,测序结果在NCBI中进行BLASTn比对,根据比对相似性判定eaeA基因的型别。将获得型别的序列与GenBank中肠致病性大肠埃希菌(Enteropathogenic Escherichia coli, EPEC)和肠出血性大肠埃希菌(Enterohemorrhagic Escherichia coli, EHEC)代表性菌株型别的序列用Neighbor-joining法构建系统发生树,分析其进化关系。 结果 所有EHEC分离株的eaeA基因均为γ型,且序列完全一致。EPEC分离株的eaeA基因型以β1型为主,同时存在ε, κ, ι2, θ型。 结论 EHEC分离株的eaeA基因相对保守,型别单一,而EPEC分离株的eaeA基因表现出较大的多态性。
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| 关 键 词： | 大肠埃希菌 eaeA基因 分型 |
| 收稿时间： | 2011-11-18 |
Allelic subtyping of eaeA of Escherichia coli(EHEC/EPEC) isolates |
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| CHEN Qiang , LU Shan , ZHAO Ai-lan , XIONG Yan-wen. Allelic subtyping of eaeA of Escherichia coli(EHEC/EPEC) isolates[J]. Disease Surveillance, 2012, 27(2): 93-96 |
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| Authors: | CHEN Qiang LU Shan ZHAO Ai-lan XIONG Yan-wen |
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| Affiliation: | .State Key Laboratory for Communicable Disease Control and Prevetnion,Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China |
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| Abstract: | Objective To understand the genotypes of the eaeA gene of Escherichia coli(EHEC/EPEC)isolates in China.Methods The eaeA genes of 60 isolates of Escherichia coli were amplified by using polymerase chain reaction(PCR).The products were sequenced and analyzed with BLASTn,and the subtype was identified according to their similarity.Phylogenetic tree was constructed based on the eaeA gene sequences.Results Genotype γ was found in the eaeA genes,with identical sequences,of all 43 EHEC isolates,however,the genotypes of eaeA genes were diverse in 17 EPEC isolates and β1 was the most common genotype(70.6%),followed by κ(11.6%),ε(5.8%),ι2(5.8%)and θ(5.8%).Conclusion Based on the sequence analysis of intimin gene family,we found genetic diversity in EPEC isolates and genetic conservativeness in EHEC isolates. |
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| Keywords: | Escherichia coli eaeA gene typing |
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