| 补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究 |
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| 引用本文： | 贺文彬,张俊龙,陈乃宏,张岭,朱海波. 补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究[J]. 中国药学, 2005, 14(2): 119-124 |
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| 作者姓名： | 贺文彬 张俊龙 陈乃宏 张岭 朱海波 |
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| 作者单位： | [1]山西中医学院，山西太原030024 [2]中国医学科学院中国协和医科大学药物研究所，北京100050 |
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| 基金项目： | 山西省自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的运用中药血清药理学方法研究补肾复方含药血清(BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用.方法以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用,采用流式细胞仪观察BS对谷氨酸所诱导PC12细胞凋亡的影响.结果 BS含药血清可以增强PC12细胞活力,拮抗谷氨酸的兴奋性神经毒作用且在20%浓度时作用最强,并且能够抑制谷氨酸诱导的PC12细胞早期凋亡.结论补肾方BS可能具有神经保护作用.
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| 关 键 词： | 补肾复方 神经保护 细胞凋亡 中药血清药理学 PC12细胞 |
Neuroprotective Effects of Bushen Decoction Against Glutamate-Induced Neurotoxicity in PC12 Cells |
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| He Wen-bin,ZHANG Jun-long,CHEN Nai-hong,ZHANG ling,ZHU Hai-Bo. Neuroprotective Effects of Bushen Decoction Against Glutamate-Induced Neurotoxicity in PC12 Cells[J]. Journal of Chinese Pharmaceutical Sciences, 2005, 14(2): 119-124 |
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| Authors: | He Wen-bin ZHANG Jun-long CHEN Nai-hong ZHANG ling ZHU Hai-Bo |
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| Abstract: | Aim The enhanced effect of Bushen (Kidney-tonifying) decoction (BS) on cultured PC12 cell proliferation and its antagonistic action on neurotoxicity induced by glutamate were investigated by serum pharmacological method of the Chinese material medica (CMM) in vitro. Methods The effect of BS on cultured PC12 cell activity and its antagonistic action on neurotoxicity induced by glutamate was observed by MTT method. Flow cytometry and fluorescence microscope techniques were employed to observe the antagonistic effect of BS on early period apoptosis of PC12 cells induced by glutamate. Results The serum with BS was able to enhance activity of PC12 cells and exert antagonistic effect on glutamate-induced neurotoxicity. Meanwhile, these beneficial effects produced by BS were found to be the strongest in 20% concentration of in serum BS. Moreover, it can inhibit apoptosis of PC12 cells induced by glutamate, which occurs in the early period.Conclusion BS may exert a potential neuroprotective effect. |
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| Keywords: | Bushen decoction neuroprotective effect apoptosis pharmacology Chinese material medica PC12 cells |
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