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| 中国南方食管鳞癌APC基因连锁位点D5S346和D5S82杂合性丢失的研究 | |
| 引用本文： | 李小东,胡祎,甘德秀,马国伟,苏晓东,王军业,张兰军,傅剑华,戎铁华. 中国南方食管鳞癌APC基因连锁位点D5S346和D5S82杂合性丢失的研究[J]. 实用医学杂志, 2007, 23(19): 2992-2994 |
| 作者姓名： | 李小东 胡祎 甘德秀 马国伟 苏晓东 王军业 张兰军 傅剑华 戎铁华 |
| 作者单位： | 1. 中山大学肿瘤防治中心胸科,华南肿瘤学国家重点实验室,广州市,510060 2. 中山大学公共卫生学院,广州市,510089 |
| 基金项目： | 国家自然科学基金;广东省自然科学基金;面向21世纪教育振兴行动计划(985计划) |
| 摘 要： | 目的:分析中国南方食管癌高发地区(潮汕地区)食管鳞状细胞癌APC基因连锁位点D5S346和D5S82的杂合性丢失情况。方法:采用PCR银染技术,检测42例配对食管鳞癌标本D5S346和D5S82的杂合性丢失情况。结果: D5S346和D5S82在该食管癌人群中杂合性丢失率高达35%和39%。结论:APC基因附近微卫星标记D5S346和D5S82在中国南方食管鳞癌中的杂合性丢失频率较高,提示APC基因在食管癌中可能同样存在缺失改变。 |
| 关 键 词： | 食管肿瘤 基因 APC 微卫星重复 杂合性丢失 |
| 修稿时间： | 2007-06-21 |
Loss of heterozygosity with microsatellite markers close to APC gene of esophageal squamous cell carcinoma in South China |
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| LI Xiao-dong,HU Yi,GAN De-xiu,MA Guo-wei,SU Xiao-dong,WANG Jun-ye,ZHANG Lan-jun,FU Jian-hua,RONG Tie-hua. Loss of heterozygosity with microsatellite markers close to APC gene of esophageal squamous cell carcinoma in South China[J]. The Journal of Practical Medicine, 2007, 23(19): 2992-2994 | |
| Authors: | LI Xiao-dong HU Yi GAN De-xiu MA Guo-wei SU Xiao-dong WANG Jun-ye ZHANG Lan-jun FU Jian-hua RONG Tie-hua |
| Affiliation: | Thoracic Department, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, China |
| Abstract: | Objective To analyze loss of heterozygosity(LOH) with microsatellite markers D5S346 and D5S82 close to APC gene of esophageal squamous cell carcinoma(ESCC) in high incidence area of South China(Chaoshan area).Method Polymerase chain reaction(PCR) based-on LOH was performed in 42 primary ESCC with 5 microsatellite markers.Result The frequencies of allelic loss of D5S346 and D5S82 were 35% and 39%,respectively.Conclusion The frequencies of LOH at the locus D5S346 and D5S82 are increased,suggesting that APC gene deletion may also occur in ESCC. |
| Keywords: | Esophageal neoplasms Genes APC Microsatellite repeats Loss of heterozygosity |
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