| 人持续性G病毒Ⅱ型在献血者和反复接受输血患者中的检测与意义 |
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| 引用本文： | 朱娜玲,许如,唐伟平,王海鹰,万政伟,吴学东,付涌水,唐时幸,俞守义. 人持续性G病毒Ⅱ型在献血者和反复接受输血患者中的检测与意义[J]. 南方医科大学学报, 2018, 38(7): 842 |
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| 作者姓名： | 朱娜玲 许如 唐伟平 王海鹰 万政伟 吴学东 付涌水 唐时幸 俞守义 |
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| 摘 要： | 目的确定我国献血者、反复接受输血患者HPgV-2感染率，阐明HPgV-2对血液安全的可能影响。方法运用血清学、反转录聚合酶链反应（RT-PCR）等方法分别在1060份健康献血者、1402份HCV、500份HBV、570份反复接受输血者、248份血友病患者共3780份血清标本中检测抗HPgV-2抗体和HPgV-2 RNA，对获得的HPgV-2 NS3片段和HPgV-2全基因组进行核酸序列测定和分子进化分析。同时检测献血者、反复接受输血者和血友病患者血清丙型肝炎病毒（HCV）和乙型肝炎病毒（HBV）感染情况辅助作为实验结果。结果（1）感染HCV（RNA+/Ab+）的献血者中抗HPgV-2抗体阳性率为1.21%（17/1402），核酸阳性率为0.36%（5/1402），而其他几组人群中几乎未检出HPgV-2 病毒，这提示HPgV-2 感染可能主要与HCV密切相关（χ2=13.78，P=0.004）；（2）5株HPgV-2病毒保守区域NS3区同源性达97.11%，与国外分离毒株比较，其同源性为96.53%，未发现明显变异株。结论健康献血者中HPgV-2的感染率很低，HPgV-2主要和HCV共感染，反复接受输血患者和血友病患者没有发现HPgV-2感染，提示HPgV-2经输血传播不常见，可能对血液安全影响不明显。
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Detection of a novel human pegivirus HPgV-2 in healthy blood donors and recipients ofmultiple transfusions: implications for blood safety |
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| Abstract: | Objective To investigate the prevalence of HPgV-2 in blood donors, transfusion recipients and hemophilia patientsand its impact on blood safety. Methods Serum samples were collected from 1060 healthy blood donors, 1402 HCV-positiveand 500 HBV- positive blood donors, 570 transfusion recipients and 248 hemophilia patients for screening anti-HPgV-2antibodies, HPgV-2 RNA, anti-HCV and HBsAg/HBV-DNA using ELISA and RT-PCR. Phylogenetic analysis of near fulllengthgenome sequences and NS3 genes of pegiviruses and hepaciviruses were performed using MEGA software. ResultsAnti-HPgV-2 positivity and HPgV-2 RNA positivity were found in 1.21% (17/1402) and 0.36% (5/1402) of the blood donorsinfected with HCV (RNA+/Ab+), respectively, indicating a close correlation between HPgV-2 and HCV infection (χ2=13.78, P=0.004). Anti-HPgV-2 antibody was hardly detected in the other populations. A nucleotide identity as high as 97.11% was foundin the NS3 fragments among the 5 isolated HPgV-2 strains, which had a nucleotide identity of 96.53% with the reported strainsisolated out of China. Conclusion The prevalence of HPgV-2 infection is rather low in healthy blood donors and transfusionrecipients. Coinfection with HCV is common in HPgV-2 infection, and no evidence has now been available to support HPgV-2transmission via blood transfusion, indicating that HPgV-2 may not pose a threat to blood safety. |
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