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| 缺血预处理对心肌细胞超微结构及CX43蛋白表达的保护作用 | |
| 引用本文： | 庄梅,罗红鹤,刘晓冰,陈振光. 缺血预处理对心肌细胞超微结构及CX43蛋白表达的保护作用[J]. 中山大学学报(医学科学版), 2006, 27(5): 562-565 |
| 作者姓名： | 庄梅 罗红鹤 刘晓冰 陈振光 |
| 作者单位： | 1. 中山大学附属第一医院保健门诊,广东,广州,510080 2. 中山大学附属第一医院胸外科,广东,广州,510080 3. 深圳孙逸仙心血管医院,广东,深圳,518001 |
| 摘 要： | 【目的】观察缺血预处理对缺血/再灌注心肌细胞超微结构及细胞连接蛋白43(Connexin43,CX43)表达的保护作用。【方法】连续观察54例心瓣膜病接受瓣膜置换的病人,随机分为缺血预处理组和对照组(非缺血预处理)。体外循环前后取右心耳组织标本处理,电子显微镜下观察心肌细胞超微结构的变化,免疫组化方法测定心肌细胞纤维CX43蛋白表达水平,并作图像定量分析。对比手术前后超声心动图的心功能指标。【结果】缺血/再灌注后,缺血预处理组心肌细胞线粒体和闰盘结构基本完整,对照组心肌细胞超微结构破坏严重。手术前两组间心肌细胞CX43表达无明显差异。缺血预处理组术前后CX43表达无明显变化(P>0.05),而对照组术后明显降低(P<0.05)。术后两组心肌细胞CX43表达有显著性差异(P<0.05)。超声心动图测定左心室射血分数和短轴缩短率,缺血预处理组术前后比较无显著性变化,对照组术后左心室射血分数和短轴缩短率均降低,而短轴缩短率明显低于缺血预处理组(P<0.05)。【结论】缺血预处理能维持缺血/再灌注后心肌细胞CX43蛋白表达的稳定,保护心肌细胞超微结构的完整性,有利于术后心功能恢复。 |
| 关 键 词： | 心肌保护 心功能,缺血预处理 |
| 文章编号： | 1672-3554(2006)05-0562-04 |
| 修稿时间： | 2006-02-25 |
Protection Effect of Ischemia Preconditioning on Myocardial Cellular Ultrastructure and Expression of CX43 Protein |
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| ZHUANG Mei,LUO Hong-he,LIU Xiao-bing,CHEN Zhen-guang. Protection Effect of Ischemia Preconditioning on Myocardial Cellular Ultrastructure and Expression of CX43 Protein[J]. Journal of Sun Yatsen University(Medical Sciences), 2006, 27(5): 562-565 | |
| Authors: | ZHUANG Mei LUO Hong-he LIU Xiao-bing CHEN Zhen-guang |
| Abstract: | [Objective] To study the protection effect of ischemia preconditioning (IP) on the myocardial cellular ultrastructure and expression of myocardial connexin 43 (CX43) protein before and after ischemia/reperfusion. [Methods] Continuous 54 cases of heart valve replacement were involved in the study. IP group and control group (non IP) were divided randomly. Right atrial appendage specimen was taken and processed for the following tests in each case. Morphologic analysis of myocardial ultrastructure was processed under electronmicroscope. The expression of CX43 protein in myocardial cell was tested by an immunohistochemical approach with a quantitative imaging system. Pre- and post-operative cardiac function by echocardiogram were compared. [Results] After ischemia/reperfusion, myocardial cellular mitochondria and intercalary disc of IP group were in almost normal morphology. However, identified disorganization of myocardial cellular ultrastructure could be seen in the control. There were no statistical differences of CX43 expression in myocardial cells in the two groups before operations. There were no differences of CX43 levels in IP group pre-and post operation. But it was notably lower in the control and showed statistical difference comparing with IP group(P< 0.05). For the ejection factor (EF) and the shortening factor (SF) of the left ventricle by echocardiography, there were no differences between pre- and post-operation in IP group. It appeared that EF and SF reduced in the control after operation. There was a statistical difference in SF of the control comparing with IP group(P< 0.05).[Conclusion] IP could retain the expression of CX43 protein in myocardial cell after ischemia/reperfusion, and maintain morphologic integrity of cellular ultrastructure. It is benefificial to the recovery of heart function after surgery. |
| Keywords: | CX43 |
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