| HMGN2在牙周炎菌斑生物膜形成中的作用 |
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| 引用本文： | 骆 洋,李 倩,樊亚平,等. HMGN2在牙周炎菌斑生物膜形成中的作用[J]. 四川大学学报(医学版), 2016, 47(4): 491-494 |
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| 作者姓名： | 骆 洋 李 倩 樊亚平 等 |
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| 作者单位： | 1.口腔疾病研究国家重点实验室（四川大学），四川大学华西口腔医学院 |
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| 摘 要： | 目的 研究牙周炎患者唾液高迁移率非组蛋白N2（HMGN2）含量与牙周炎相关性以及其在牙菌斑生物膜形成中的作用。方法 纳入100例参与者，分为健康组、轻度牙周炎组、中度牙周炎组和重度牙周炎组，每组25例，采用酶联免疫吸附分析法（ELISA）检测各组唾液中HMGN2的质量浓度。重组人HMGN2蛋白提纯和鉴定后，K-B法检测重组人HMGN2对牙周炎主要致病菌牙龈卟啉单胞菌的抑菌作用，结晶紫染色法检测重组人HMGN2对牙龈卟啉单胞菌生物膜形成的影响。结果 与健康组相比，牙周炎患者（尤其是重度牙周炎患者）唾液中HMGN2升高（ P<0.01），HMGN2质量浓度与患者牙菌斑指数呈负相关( r=-0.363， P<0.05)。重组人HMGN2能有效抑制牙周致病菌生长和菌斑生物膜形成( P<0.05)。结论 HMGN2参与了牙周炎菌斑生物膜的形成，在牙周炎发病过程中发挥重要作用。
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| 关 键 词： | 牙周炎 高迁移率非组蛋白N2 唾液 牙龈卟啉单胞菌 |
STThe Role of HMGN2 in the Development of Periodontitis Dental Plaque |
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| LUO Yang,LI Qian,FAN Ya-ping,et al. STThe Role of HMGN2 in the Development of Periodontitis Dental Plaque[J]. Journal of Sichuan University. Medical science edition, 2016, 47(4): 491-494 |
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| Authors: | LUO Yang LI Qian FAN Ya-ping et al |
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| Abstract: | Objective To determine the role of high mobility group chromosomal protein N2 (HMGN2) in the development of periodontitis plaque biofilm. Methods Saliva samples were collected before clinical interventions in patients with periodontitis (25 Mild periodontitis, 25 Moderate periodontitis and 25 Severe periodontitis) and healthy controls ( n=25).Following an estimation of dental plaque index,the level of HMGN2 in saliva was determined by enzyme-linked immunosorbent assay (ELISA).The recombinant human HMGN2 protein was purified and tested, its inhibitive effects on Porphyromonas gingivalis (P.g)growth and formation of P.g bioflim were detected by K-B antibacterial annulus and crystal violet staining, respectively. Results The healthy controls had lower levels of HMGN2 in saliva than those with periodontitis,especially those with severe periodontitis ( P<0.01).The level of HMGN2 was negatively correlated with dental plaque index ( r=-0.363, P<0.05). HMGN2 inhibited the development of main periodontal bacteria biofilm ( P<0.05). Conclusion HMGN2 is involved in the development of periodontitis plaque biofilm and plays an important role in the development of periodontitis. |
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| Keywords: | Periodontitis High mobility group chromosomal protein N2 Salivary Porphyromonas gingivalis |
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