| 三羟基异黄酮对关节软骨细胞合成胶原和蛋白多糖的影响 |
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| 引用本文： | 张超,陈飞雁,夏军,姜建元,黄煌渊. 三羟基异黄酮对关节软骨细胞合成胶原和蛋白多糖的影响[J]. 复旦学报(医学版), 2009, 36(4): 440-444. DOI: |
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| 作者姓名： | 张超 陈飞雁 夏军 姜建元 黄煌渊 |
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| 作者单位： | 复旦大学附属华山医院骨科,上海,200040;复旦大学附属华山医院骨科,上海,200040;复旦大学附属华山医院骨科,上海,200040;复旦大学附属华山医院骨科,上海,200040;复旦大学附属华山医院骨科,上海,200040 |
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| 摘 要： | 目的 通过对体外培养软骨细胞的实验研究,探寻受体酪氨酸激酶抑制剂对软骨细胞在细胞分子水平的影响及其可能机制。方法 对软骨细胞进行原代及传代培养,通过对培养细胞形态学及甲苯胺蓝染色观察,选择适合传代软骨细胞,应用三羟基异黄酮干预后1、2、3、4天RT-PCR、ICC及甲苯胺蓝染色观察法比较药物干预对体外培养软骨细胞Ⅰ、Ⅱ型胶原的转录和翻译水平及蛋白多糖的表达变化。结果 20 μg/mL及25 μg/mL三羟基异黄酮干预可以抑制Ⅰ型胶原表达,同时增加Ⅱ型胶原及蛋白多糖的表达。结论 三羟基异黄酮可以抑制脂多糖刺激诱导的软骨细胞反分化,对体外培养关节软骨细胞的正常表型具有一定的保护作用,其保护作用与三羟基异黄酮浓度呈现一定的相关性。
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| 关 键 词： | 蛋白多糖 Ⅰ Ⅱ型胶原 受体酪氨酸激酶抑制剂 |
| 收稿时间： | 2008-08-26 |
The effect of receptor tyrosine kinase inhibitor genistein on phenotype expression of collagen and proteoglycan of chondrocyte |
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| ZHANG Chao,CHEN Fei-yan,XIA Jun,JIANG Jian-yuan,HUANG Huang-yuan. The effect of receptor tyrosine kinase inhibitor genistein on phenotype expression of collagen and proteoglycan of chondrocyte[J]. Fudan University Journal of Medical Sciences, 2009, 36(4): 440-444. DOI: |
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| Authors: | ZHANG Chao CHEN Fei-yan XIA Jun JIANG Jian-yuan HUANG Huang-yuan |
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| Affiliation: | Department of Orthopaedics;Huashan Hospital;Fudan University;Shanghai 200040;China |
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| Abstract: | Objective To explore the protective role of the receptor tyrosine kinase inhibitor in chondrocyte in vitro.MethodsReverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry (ICC) and toluidine blue staining were used to understand the changes of phenotype expression of collagenⅠ, Ⅱ and proteoglycan in chondrocyte in vitro. Results Genistein of 20 μg/mL and 25 μg/mL concentrations decreased the expression of collagen Ⅰ and increased the expression of collagen Ⅱ and proteoglycan (P< 0. 05). Conclusions This study showes that genistein can effectively inhibit the expression of collagen Ⅰ above certain concentration, and it can improve the expression of collagen Ⅱ and increase the synthesis of proteoglycan. |
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| Keywords: | proteoglycan collagen receptor tyrosine kinase inhibitor |
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