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| 阿魏酸对视网膜色素变性小鼠血浆内皮素-1表达的影响 | |
| 引用本文： | 孙晓伟,殷晓贝,崔卉,李根林. 阿魏酸对视网膜色素变性小鼠血浆内皮素-1表达的影响[J]. 眼科新进展, 2015, 0(8): 701-703. DOI: 10.13389/j.cnki.rao.2015.0192 |
| 作者姓名： | 孙晓伟 殷晓贝 崔卉 李根林 |
| 作者单位： | 100730 北京市首都医科大学附属北京同仁医院北京同仁眼科中心 北京市眼科学与视觉科学重点实验室 |
| 摘 要： | 目的 研究不同浓度阿魏酸作用下，出生后不同时期的视网膜色素变性（ｒｅｔｉｎｉｔｉｓｐｉｇｍｅｎｔｏｓａ，ＲＰ）动物模型（ｒｄ小鼠）血浆中内皮素－１（ｅｎｄｏｔｈｅｌｉｎ－１，ＥＴ－１）动态表达特征，探讨阿魏酸对ｒｄ小鼠表达ＥＴ－１的作用特点。方法 取新出生的ｒｄ小鼠９０只及Ｃ５７／ＢＬ６小鼠１８只，按照阿魏酸灌胃浓度的不同分成４组，０．２５ｇ? Ｌ－１组、０．５０ｇ? Ｌ－１组、０．７５ｇ?Ｌ－１组、１．００ｇ?Ｌ－１组，每组再按照给药时间分为２周组、３周组、４周组，以上１２组为药物处理组，每组各６只。相同周龄的ｒｄ小鼠各６只不作处理作为病变对照组，相同周龄的Ｃ５７／ＢＬ６小鼠各６只作为正常对照组。ＥＬＩＳＡ法检测血浆ＥＴ－１浓度。结果 ２周组、３周组、４周组病变对照组小鼠ＥＴ－１浓度均高于正常对照组，尤其３周组、４周组与正常对照组相比差异有统计学意义（Ｐ＝０．０２４、０．０１０）。经阿魏酸治疗后２周时，０．５０ｇ?Ｌ－１组、０．７５ｇ?Ｌ－１组ＥＴ－１水平低于病变对照组，但差异无统计学意义；３周时，０．２５ｇ?Ｌ－１组、０．５０ｇ?Ｌ－１组、０．７５ｇ?Ｌ－１组ＥＴ－１水平低于病变对照组，其中０．５０ｇ?Ｌ－１组差异有统计学意义（Ｐ＝０．０３４）；４周时，０．２５ｇ?Ｌ－１组、０．５０ｇ?Ｌ－１组、０．７５ｇ?Ｌ－１组ＥＴ－１水平低于病变对照组，差异均有统计学意义（Ｐ＝０．０１１、０．０２７、０．０２１）。各浓度治疗组中，仅１．００ｇ?Ｌ－１组治疗３周和４周时与正常对照组相比差异有统计学意义（Ｐ＝０．０１３、０．００９）。结论 ｒｄ小鼠在病变过程中，血浆ＥＴ－１水平明显升高，可能与ＲＰ的发生发展有关。在不同浓度阿魏酸治疗下，ｒｄ小鼠ＥＴ－１水平不同程度下降，其中０．５０ｇ?Ｌ－１、０．７５ｇ?Ｌ－１治疗效果最明显。 |
| 关 键 词： | 阿魏酸 视网膜色素变性 内皮素-1 rd小鼠 |
Effects of ferulic acid on serum endothelin-l of RP mouse |
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| SUN Xiao-Wei,YIN Xiao-Bei,CUI Hui,LI Gen-Lin. Effects of ferulic acid on serum endothelin-l of RP mouse[J]. Recent Advances in Ophthalmology, 2015, 0(8): 701-703. DOI: 10.13389/j.cnki.rao.2015.0192 | |
| Authors: | SUN Xiao-Wei YIN Xiao-Bei CUI Hui LI Gen-Lin |
| Affiliation: | Beijing Tongren Eye Center, Beijing Tongren Hospital,Capital Medical University,Beijing Ophthalmology & Visual Sciences Key Lab, Beijing 100730，China |
| Abstract: | Objective To investigate the effects of ferulic acid on the serum endothelin-l of rd mouse following gavage administration of ferulic acid ( FA) . Methods A total of 90 newborn rd mice were divided into the control group and four gavage administration groups (0. 25 g . L-l group, 0. 50 g . L-l group, 0. 75 g . L-l group and 1. 00 g . L-l group) , and 18 C57/BL6 mice were included in the normal control group. The gavage adnunistration groups were divided int0 2-week group , 3-week group and 4-week group based on the gavage administration time.6 cases in each group , and ELISA was performed to detect ET-I in serum. Results ET-I levels in 2-week group, 3-week group and 4-week group were significantly higher than that in normal control group , especially the 2-week group , 3-week group ( P = 0. 024 , 0. 010 ) . After treated with different concentrations of ferulic acid for 2 weeks. ET-I levels in 0. 50 g . L -l group, 0. 75 g . L -l group were lower than that in diseased control group ; After treated for 3 weeks,ET-I levels in 0. 25 g . L-l group, 0. 50 g . L-l group,0. 75 g . L-l group were lower than that in diseased control group, and there was statistical difference in 0. 50 g . L -l group (P = 0. 034 ) ;After treated for 4 weeks , ET-I levels in 0. 25 g . L -l group , 0. 50 g . L-l group ,0. 75 g . L-l group were lower than that in diseased control group, and there were statistical difference (P = 0. 011 , 0. 027 .0. 021 ) . There were statistical differences in ET-I levels in l. 00 g . L-l group for 3 weeks and 4 weeks compared with normal control group ( P = 0. 013 .0. 009) . Coriclrision ET-I levels in mice plasma rncrease significantly in the process of pathological changes of rd mouse , and may be associated with the development of RP. Under the different concentrations of ferulic acid treatment . ET-I levels significantly decrease. The effects are obvious in 0. 50 g . L -1 and 0. 75 g . L-1 concentrations. |
| Keywords: | ferulic acid retinitis pigmentosa endothelin-l rd mouse |
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