| 辐射诱发胸腺淋巴瘤c-mye基因启动子甲基化检测 |
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| 引用本文： | 于雷, 刘永哲, 孙世龙, 方芳, 巩宏伟, 陈强, 鞠桂芝. 辐射诱发胸腺淋巴瘤c-mye基因启动子甲基化检测[J]. 中国公共卫生, 2011, 27(1): 81-82. DOI: 10.11847/zgggws2011-27-01-39 |
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| 作者姓名： | 于雷 刘永哲 孙世龙 方芳 巩宏伟 陈强 鞠桂芝 |
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| 作者单位： | 1.吉林大学第二医院放疗科, 长春 130021;2.吉林大学公共卫生学院卫生部放射生物学重点实验室 |
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| 基金项目： | 国家自然科学基金，吉林大学研究生创新基金资助项目 |
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| 摘 要： | 目的 研究辐射诱发小鼠胸腺淋巴瘤c-mcy基因启动子CpG岛甲基化状态和mPNA表达的变化.方法 X射线照射BALB/c小鼠建立胸腺淋巴瘤模型,采用硫化测序PCR(bisulfite sequencing PCR,BCP)法和逆转录-聚合酶链反应(RT-PCR)分别检测c-myc基因启动子CpG岛甲基化状态和mRNA的表达.结果 辐射诱发胸腺淋巴瘤c-myc基因启动子CpG岛与正常胸腺组织比较呈去甲基化状态,RT-PCR检测结果显示,胸腺淋巴瘤c-myc基因mPNA相对表达量(1.12±0.99)明显高于正常胸腺组织(0.89±0.08),2组比较差异有统计学意义(P<0.01).结论 c-myc基因启动子去甲基化可能与辐射诱发胸腺淋巴瘤密切有关.
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| 关 键 词： | 胸腺淋巴瘤 甲基化 硫化测序PCR CpG岛 |
| 收稿时间： | 2010-05-18 |
Detection on promoter methylation of c-myc gene in thymic lymphomas induced by ionizing radiation |
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| YU Lei, LIU Yong-zhe, SUN Shi-long, . Detection on promoter methylation of c-myc gene in thymic lymphomas induced by ionizing radiation[J]. Chinese Journal of Public Health, 2011, 27(1): 81-82. DOI: 10.11847/zgggws2011-27-01-39 |
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| Authors: | YU Lei LIU Yong-zhe SUN Shi-long |
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| Affiliation: | 1.Department of Radiotherapy, Second Hospital, Jilin University Changchun 130021, China |
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| Abstract: | Objective To study methy lation of CpGislands in prom oter region of c-mcy gene and the change of mRNA expression in thymic lymphomas of mice induced byionizing radiation.Methods The thymic lymphom as model was made with X-ray exposure in BAL B/c mice.The methylation of CpG islands in prom oter region and the mRNA expression of c-myc gene were detected with bisulfite sequencing PCR and RT-PCR.Results CpG islands inprom oter region of c-myc gene were at demethy lation state in thymic lymhom as induced by ionizing radiation against normal thymus.The mRNA expression of c-myc gene in thymic lymphomas(1.12±0.99) was higher than that of in normal thym us tissue(0.89±0.08) with a tatistical sign if icance(P < 0.01).Conclusion There is a corre lation between promoter demethylation of c-myc gene and thymiclym phom as induced by ionizing radiation. |
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| Keywords: | thymic lym phoma mthy lation bisulfite sequencing PCR CgGisland |
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