Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization |
| |
| Authors: | Traidl-Hoffmann Claudia Mariani Valentina Hochrein Hubertus Karg Kathrin Wagner Hermann Ring Johannes Mueller Martin J Jakob Thilo Behrendt Heidrun |
| |
| Affiliation: | 1.ZAUM-Center for Allergy and Environment, Division of Environmental Dermatology and Allergy GSF/TUM; 2.Institute of Medical Microbiology, Immunology and Hygiene; 3.Department of Dermatology and Allergy Biederstein, Technische, Universität München, 80802 Munich, Germany; 4.Julius-von-Sachs-Institute of Biosciences, Division of Pharmaceutical Biology, University of Würzburg, 97082 Würzburg, Germany |
| |
| Abstract: | Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved in allergic inflammation such as neutrophils and eosinophils. Here we demonstrate that soluble factors from birch (Betula alba L.) pollen activate human dendritic cells (DCs) as documented by phenotypical and functional maturation and altered cytokine production. Betula alba L. aqueous pollen extracts (Bet.-APE) selectively inhibited interleukin (IL)-12 p70 production of lipopolysaccharide (LPS)- or CD40L-activated DC, whereas IL-6, IL-10, and TNFalpha remained unchanged. Presence of Bet.-APE during DC activation resulted in DC with increased T helper type 2 (Th2) cell and reduced Th1 cell polarizing capacity. Chemical analysis of Bet.-APE revealed the presence of phytoprostanes (dinor isoprostanes) with prostaglandin E(1)-, F(1)-, A(1)-, or B(1)-ring systems of which only E(1)-phytoprostanes dose dependently inhibited the LPS-induced IL-12 p70 release and augmented the Th2 cell polarizing capacity of DC. These results suggest that pollen-derived E(1)-phytoprostanes not only resemble endogenous prostaglandin E(2) structurally but also functionally in that they act as regulators that modulate human DC function in a fashion that favors Th2 cell polarization. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
