| UBE2 O在胃癌组织中的表达及其对胃癌细胞增殖和侵袭的影响 |
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| 引用本文： | 党立力,刘畅,周华友,周鹏,张流,余钧辉,赵伟. UBE2 O在胃癌组织中的表达及其对胃癌细胞增殖和侵袭的影响[J]. 现代肿瘤医学, 2022, 0(3): 465-470. DOI: 10.3969/j.issn.1672-4992.2022.03.018 |
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| 作者姓名： | 党立力 刘畅 周华友 周鹏 张流 余钧辉 赵伟 |
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| 作者单位： | 1.汉中市中心医院普通外科，陕西 汉中 723000;2.西安交通大学第一附属医院普通外科，陕西 西安 710061 |
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| 基金项目： | 国家自然科学基金资助项目(编号:82002547)。 |
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| 摘 要： | 目的:研究泛素结合酶E2O(ubiquitin conjugating enzyme E2 O,UBE2O)在胃癌组织中的表达及临床意义,明确UBE2O对胃癌细胞增殖和侵袭的影响,并初步探索潜在分子机制.方法:利用癌症基因图谱(The Cancer Genome Atlas,TCGA)数据库分析UEB2O mRNA在胃...
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| 关 键 词： | 泛素连接酶E2O 胃癌 增殖 侵袭 AMP活化蛋白激酶α2 雷帕霉素机制靶点 |
UBE2O expression in gastric cancer tissues and its effects on gastric cancer cell proliferation and invasion |
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| DANG Lili,LIU Chang,ZHOU Huayou,ZHOU Peng,ZHANG Liu,YU Junhui,ZHAO Wei. UBE2O expression in gastric cancer tissues and its effects on gastric cancer cell proliferation and invasion[J]. Journal of Modern Oncology, 2022, 0(3): 465-470. DOI: 10.3969/j.issn.1672-4992.2022.03.018 |
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| Authors: | DANG Lili LIU Chang ZHOU Huayou ZHOU Peng ZHANG Liu YU Junhui ZHAO Wei |
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| Affiliation: | 1.Department of General Surgery,Hanzhong Central Hospital,Shaanxi Hanzhong 723000,China;2.Department of General Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China. |
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| Abstract: | Objective:To study the expression and clinical significance of ubiquitin conjugating enzyme E2 O(UBE2O)in gastric cancer,clarify the effect of UBE2O on the proliferation and invasion of gastric cancer cells and explore the potential molecular mechanisms.Methods:The Cancer Genome Atlas(TCGA)database was used to analyze the expression differences of UBE2O mRNA in gastric cancer and normal tissues.Immunohistochemical staining was used to clarify the expression of UBE2O protein in gastric cancer and adjacent tissues.AGS and SGC-7901 cells were transfected with UBE2O shRNA.CCK-8 and transwell assays were used to detect cell proliferation and invasion ability.Western blotting assay was carried out to detect the expression of AMP-activated protein kinaseα2(AMPKα2),phosphorylated mechanistic target of rapamycin(p-mTOR)and mTOR.Results:TCGA data indicated that the expression of UBE2O mRNA in gastric cancer tissues was significantly higher than that in normal tissues.Immunohistochemical staining results confirmed that the positive expression rate of UBE2O in gastric cancer tissues was prominently higher than that in adjacent tissues.UBE2O expression was significantly correlated with tumor size,depth of invasionand TNM stage.UBE2O knockdown markedly inhibited the proliferation and invasion of AGS and SGC-7901 cells.UEB2O silencing resulted in an increase in AMPKα2 protein level,while a decrease in p-mTOR protein level in AGS and SGC-7901 cells.Conclusion:UBE2O is up-regulated in gastric cancer tissues and is closely related to malignant clinicopathological characteristics.UBE2O may promote the proliferation and invasion of gastric cancer cells through AMPKα2/mTOR signaling pathway. |
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| Keywords: | ubiquitin conjugating enzyme E2 O gastric cancer proliferation invasion AMP-activated protein kinaseα2 mechanistic target of rapamycin |
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