| 胆管癌组织紧密连结蛋白18、丝氨酸蛋白酶抑制剂和p53蛋白表达及其临床意义 |
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| 引用本文： | 曹光材,白江江,戴阿利. 胆管癌组织紧密连结蛋白18、丝氨酸蛋白酶抑制剂和p53蛋白表达及其临床意义[J]. 实用肝脏病杂志, 2017, 20(3): 312-315. DOI: 10.3969/j.issn.1672-5069.2017.03.014 |
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| 作者姓名： | 曹光材 白江江 戴阿利 |
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| 作者单位： | 716000陕西省延安市 延安大学附属医院肛肠外科(曹光材,白江江）; 陕西延安职业技术学院护理系（戴阿利） |
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| 摘 要： | 目的 探讨胆管癌组织紧密连结蛋白18（claudin-18）、丝氨酸蛋白酶抑制剂（maspin）和p53蛋白表达的临床意义。方法 采用免疫组化和Western blot法检测46例胆管癌和32例正常胆管组织claudin-18、maspin和p53蛋白的表达,采用Spearman等级相关分析。结果 胆管癌组织claudin-18和p53蛋白阳性率分别为52.17%和56.52%,显著高于正常胆管组织（0.0%和0.0%,P<0.01）;胆管癌组织maspin阳性率为86.96%,显著低于正常胆管组织（100.0%,P<0.01）;胆管癌组织claudin-18、maspin和p53蛋白表达量分别为2.83±0.52、2.45±1.24和1.73±1.26,均显著高于正常胆管组织（0.48±0.75、0.46±0.32和0.0±0.0,P<0.05）;胆管癌组织maspin与p53表达呈负相关（r=-8.546,P=0.001）。结论 胆管癌组织maspin、claudin-18和p53蛋白表达的意义还有待于进一步研究。
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| 关 键 词： | 胆管癌 紧密连结蛋白18 丝氨酸蛋白酶抑制剂 p53 免疫组化 蛋白印迹法 |
| 收稿时间： | 2016-09-27 |
Expression of claudin-18, maspin and p53 in tissues from cholangiocarcinoma |
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| Cao Guangcai,Bai Jiangjiang,Dai Ali. Expression of claudin-18, maspin and p53 in tissues from cholangiocarcinoma[J]. Journal of Clinical Hepatology, 2017, 20(3): 312-315. DOI: 10.3969/j.issn.1672-5069.2017.03.014 |
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| Authors: | Cao Guangcai Bai Jiangjiang Dai Ali |
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| Affiliation: | Department of Anorectal Surgery,Affiliated Hospital,Yan'an University,Yan'an 716000,Shaanxi Province,China |
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| Abstract: | Objective To study the expression of claudin-18,maspin and p53 in tissues of cholangiocarcinoma. Methods The expression of claudin-18,maspin and p53 were detected by immunohistochemistry and Western blot in 46 cholangiocarcinoma and 32 normal liver tissues. Result The positive rates of claudin-18 and p53 in cancerous tissues were 52.17% and 56.52%,respectively,significantly higher than 0.0% and 0.0% in normal control（P<0.01）;The positive rate of maspin in cancerous tissues was 86.96%,much lower than 100.0% in normal control（P<0.01）;The relative expression levels of claudin-18,maspin and p53 were 2.83±0.52, 2.45±1.24 and 1.73±1.26, respectively,significantly higher than 0.48±0.75,0.46±0.32 and 0.0±0.0 in the control （P<0.05）;The expression of maspin was negatively correlated with p53 expression in cancerous tissues（r=-8.546,P=0.001）. Conclusion The implication of maspin,claudin-18 and p53 expression in cholangiocarcinoma tissues needs further investigation. |
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| Keywords: | Cholangiocarcinoma Claudin-18 Maspin p53 Immunohistochemistry Western blot |
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