Predictors for successful PBSC collection on the fourth day of G‐CSF‐induced mobilization in allogeneic stem cell donors |
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| Authors: | Anja van Oostrum Jaap Jan Zwaginga Sandra Croockewit Jacqueline Overdevest Mirjam Fechter Bart Ruiterkamp Anneke Brand Tanja Netelenbos |
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| Affiliation: | 1. Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands;2. Centre for Clinical Transfusion Research, Sanquin, Leiden, The Netherlands;3. Department of Hematology, Radboud University Medical Centre, Nijmegen, The Netherlands;4. Matchis Foundation, Leiden, The Netherlands |
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| Abstract: | Peripheral blood stem cells (PBSCs) used for allogeneic transplantation are collected by apheresis after pre‐treatment of donors with G‐CSF. Using modern apheresis devices stem cells can be collected more efficiently. It was studied whether collection on the 4th instead of the 5th day after initiation of G‐CSF treatment might be feasible. Stem cell yields that could have been collected on day 4 were calculated in two cohorts treated with 10 µg/kg G‐CSF once daily (n = 106, cohort I) or 5 µg/kg twice daily schedule (n = 85, cohort II). Harvests were predicted using the median collection efficiency (CE) of the apheresis machine and regarded successful when > 5.0 x106 CD34+/kg recipient body weight. Successful harvests at day 4 could have been obtained in only 22.6% and 41.2% of donors in cohort I and II respectively, while the expected successful collections on day 5 were 55.7% and 76.5%. Individual donor factors that correlated with a successful harvest on day 4 were weight, BMI, age, ratio donor/recipient weight and total G‐CSF dose in cohort I, whereas ratio donor/recipient weight was the only significant predictor in cohort II. Donor weight, BMI and total G‐CSF dose correlated positively with CD34+ values in the blood on day 4 in all donors. However, donor characteristics were not able to be used as strong predictors in daily practice. In conclusion, PBSC collection on day 4 will not result in a successful harvest in most stem cell donors, however using a twice daily G‐CSF scheme increases the yield. |
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| Keywords: | apheresis G‐CSF dosage mobilization mononuclear cell collection |
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