| 浙江省检出带毒力基因的人源性肠出血性大肠杆菌O157:H7 |
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| 引用本文： | 叶菊莲,程苏云,罗芸,孟真,莫顺堂,陆群英. 浙江省检出带毒力基因的人源性肠出血性大肠杆菌O157:H7[J]. 疾病监测, 2005, 20(12): 625-628. DOI: 10.3784/j.issn.1003-9961.2005.12.625 |
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| 作者姓名： | 叶菊莲 程苏云 罗芸 孟真 莫顺堂 陆群英 |
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| 作者单位： | 1.浙江省疾病预防控制中心 310009 |
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| 摘 要： | 目的了解肠出血性大肠杆菌O157:H7在浙江省人群中的感染情况和菌型分布特征,制定相应的防治对策。方法5～10月份肠道传染病高发季节,在浙江省各地(市)肠道门诊采集腹泻病人粪便,对O157:H7菌株进行分离培养,并用PCR方法检测其毒力基因。结果1998年开始至今的人群监测中,共检出2株无毒力的肠出血性大肠杆菌O157:H7。PCR毒力基因检测,SLT2和Hly阳性,SLT1阴性。结论与以往不同,此次分离到带有毒力基因的人源性肠出血性大肠杆菌O157:H7,说明浙江省O157:H7在菌型特征方面发生了变化,对人群健康构成了更大的威胁。
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| 关 键 词： | 肠出血性大肠杆菌O157:H7 毒力 分离 |
| 文章编号： | 1003-9961（2005）12-0625-03 |
| 收稿时间： | 2005-07-19 |
| 修稿时间： | 2005-07-192005-10-10 |
Human EHEC O157: H7 with Virulence Gene Detected in Zhejiang Province |
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| YE Ju-lian,CHENG Su-yun,LUO Yun,MENG Zhen,MO Shun-tang,LU Qun-ying. Human EHEC O157: H7 with Virulence Gene Detected in Zhejiang Province[J]. Disease Surveillance, 2005, 20(12): 625-628. DOI: 10.3784/j.issn.1003-9961.2005.12.625 |
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| Authors: | YE Ju-lian CHENG Su-yun LUO Yun MENG Zhen MO Shun-tang LU Qun-ying |
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| Affiliation: | 1.Zhejiang Provincial Center for Disease Control and Prevention;Hangzhou 310009 China; |
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| Abstract: | Objective The present study was designed to know the infection status of Enterohaemorrhagic Escherichia coli, serotype O157:H7 and the distribution feature of the bacterial types in Zhejiang province to conducive to the establishment of corresponding strategies of its prevention and control. Methods Faeces of patients with diarrhea were collected at diarrhea clinics of different prefectures and cities in Zhejiang province from May to October during which enteric infectious diseases were highly prevalent. And The serotype O157:H7 strain was isolated and cultured and the virulence gene was detected by PCR. Results Two strains of Enterohaemorrhagic Escherichia coli, serotype O157:H7 were detected in the population examined from 1998 to the day when this study was completed. Detection of virulence gene by PCR revealed positive SLT2 and Hly and negative SLT1. Conclusion The human Enterohaemorrhagic Escherichia coli, serotype O157:H7 with virulence gene isolated this time is different from those observed previously, suggesting that some characters of O157:H7 have changed and these strains are more detrimental to people's health. |
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| Keywords: | Enterohaemorrhagic Escherichia coli O157:H7 Virulence Isolation |
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