Multiplex PCRs for identification of Escherichia coli virulence genes |
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| Authors: | Pass M A Odedra R Batt R M |
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| Affiliation: | Department of Physiology and Pharmacology, The University of Queensland, St. Lucia, Queensland, 4072, Australia. mpass@usc.edu.au |
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| Abstract: | PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs. |
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