Comparison of a polymer conjugate-enhanced enzyme immunoassay to ligase chain reaction for diagnosis of Chlamydia trachomatis in endocervical swabs |
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| Authors: | Chernesky M Jang D Copes D Patel J Petrich A Biers K Sproston A Kapala J |
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| Affiliation: | McMaster University and St. Joseph's Hospital, 50 Charlton Ave. East, Hamilton, Ontario L8N 4A6, Canada. chernesk@mcmaster.ca |
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| Abstract: | Two endocervical swabs from each of 1,123 women were collected into manufacturer-supplied transport tubes and tested for Chlamydia trachomatis by a polymer conjugate-enhanced (PCE) enzyme immunoassay (EIA) (IDEIA PCE Chlamydia; DAKO) and a ligase chain reaction assay (LCx Chlamydia; Abbott). After confirmation by the EIA blocking test, the sensitivity of the IDEIA PCE remained at 91.8% and the specificity increased from 98.2 to 99.8% compared to LCx. |
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