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长链非编码RNA SNHG3在食管鳞状细胞癌中的表达及其对ECA-109细胞迁移和侵袭的影响
引用本文:郭晓锋,朱曼,许广辉,王文光,郝安林. 长链非编码RNA SNHG3在食管鳞状细胞癌中的表达及其对ECA-109细胞迁移和侵袭的影响[J]. 中国肿瘤临床, 2020, 47(12): 595-600. DOI: 10.3969/j.issn.1000-8179.2020.12.413
作者姓名:郭晓锋  朱曼  许广辉  王文光  郝安林
作者单位:①.安阳市肿瘤医院胸外科(河南省安阳市 455000)
基金项目:本文课题受河南省科技攻关计划172102310116
摘    要:目的:探究长链非编码RNA SNHG3在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中的表达及其对ECA-109细胞迁移和侵袭的影响。方法:收集安阳市肿瘤医院2011年6月到2014年6月收治的60例ESCC患者癌及对应癌旁组织样本,采用qRT-PCR检测ESCC癌与癌旁组织、ESCC细胞ECA-109和人正常食管上皮细胞HEEC中lncRNA SNHG3的表达水平,分析ESCC患者组织中lncRNA SNHG3的表达与ESCC患者临床特征的关系。采用siRNA-SNHG3质粒转染ECA-109细胞,以敲降lncRNA SNHG3的表达水平。采用Kaplan-Meier法分析lncRNA SNHG3表达与患者预后的关系。采用Transwell实验检测ECA-109细胞的迁移和侵袭能力。采用qRT-PCR和Western blot实验检测ECA-109细胞中SNAIL和TWIST的mRNA及蛋白的表达水平。结果:ESCC肿瘤组织中lncRNA SNHG3的表达水平显著高于癌旁组织(P<0.05),ECA-109细胞中lncRN...

关 键 词:长链非编码RNA  SNHG3  食管鳞状细胞癌  迁移  侵袭
收稿时间:2020-01-06

Expression of long non-coding RNA SNHG3 in esophageal squamous cell carcinoma and its effect on the migration and invasion of ECA-109 cells
Xiaofeng Guo,Man Zhu,Guanghui Xu,Wenguang Wang,Anlin Hao. Expression of long non-coding RNA SNHG3 in esophageal squamous cell carcinoma and its effect on the migration and invasion of ECA-109 cells[J]. Chinese Journal of Clinical Oncology, 2020, 47(12): 595-600. DOI: 10.3969/j.issn.1000-8179.2020.12.413
Authors:Xiaofeng Guo  Man Zhu  Guanghui Xu  Wenguang Wang  Anlin Hao
Affiliation:①.Department of Thoracic Surgery, Anyang Tumor Hospital, Anyang 455000, China②.Clinical Laboratory, Anyang Tumor Hospital, Anyang 455000, China③.Department of Thoracic Surgery, Henan Province Chest Hospital, Zhengzhou 450000, China
Abstract:  Objective  To investigate the expression of long non-coding RNA(lncRNA) SNHG3 in esophageal squamous cell carcinoma (ESCC) and its effect on the migration and invasion of ECA-109 cells.  Methods  Samples of tumor and corresponding para-carcinoma tissues were collected from 60 patients with ESCC, who were enrolled in Anyang Tumor Hospital from June 2011 to June 2014. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA SNHG3 in ESCC tumor and para-carcinoma tissues, ECA-109 ESCC cells, and HEEC human normal esophageal epithelial cells. The correlation between lncRNA SNHG3 expression and clinical characteristics of patients with ESCC was assessed. ECA-109 cells were transfected with siRNA-SNHG3 plasmids to knockdown lncRNA SNHG3 expression. The correlation between lncRNA SNHG3 expression and patient prognosis was determined by Kaplan-Meier analysis. Transwell assay was used to investigate the migration and invasion abilities of ECA-109 cells. SNAIL and TWIST mRNA and protein levels in ECA-109 cells were detected by qRT-PCR and Western blot assay, respectively.  Results  The expression level of lncRNA SNHG3 was significantly higher in ESCC tumor tissues compared to that in pericarcinomatous tissues and in ECA-109 cells compared to that in HEEC cells (both P < 0.05). LncRNA SNHG3 expression in ESCC cells was significantly correlated with tumor differentiation, TNM stage, and lymph node metastasis (P < 0.05). LncRNA SNHG3 expression in ECA-109 cells decreased significantly upon transfection with the siRNA-SNHG3 plasmid (P < 0.05). High expression of lncRNA SNHG3 was significantly correlated with poor prognosis in patients with ESCC. After lncRNA SNHG3 knockdown, the migration and invasion of ECA-109 cells and the mRNA and protein expression levels of SNAIL and TWIST were reduced significantly (P < 0.05).  Conclusion  LncRNA SNHG3 is highly expressed in ESCC tumor tissues and cells and promotes the migration and invasion of ECA-109 cells by regulating the expression of SNAIL and TWIST proteins. 
Keywords:long non-coding RNA(lncRNA)  SNHG3  esophageal squamous cell carcinoma(ESCC)  migration  invasion
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