| 新城疫病毒D417株抗小细胞肺癌的体外研究 |
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| 引用本文： | 宋德志,杨建林,姜艳华,黄川,赖振屏,茅乃权,樊晓晖. 新城疫病毒D417株抗小细胞肺癌的体外研究[J]. 国际生物制品学杂志, 2008, 31(3): 97-100. DOI: 10.3760/cma.j.issn.1673-4211.2008.03.001 |
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| 作者姓名： | 宋德志 杨建林 姜艳华 黄川 赖振屏 茅乃权 樊晓晖 |
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| 作者单位： | 广西医科大学微生物学与免疫学教研室,南宁,530021 广西医科大学附属肿瘤医院 |
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| 基金项目： | 广西壮族自治区研究生教育创新计划，广西留学回国人员科学基金，教育部留学回国人员科研启动基金 |
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| 摘 要： | 目的 研究新城疫病毒(Newcastle disease virus,NDV)19417株体外对小细胞肺癌细胞NCI-H446及正常人胚肺成纤维细胞HFL-1的杀伤作用. 方法 应用四甲基偶氮唑盐比色法检测NDV D417株对NCI-H446和HFL-1的杀伤效应.D417株感染NCI-H446后,于光镜和电镜下检查细胞病理变化. 结果 NDV D417株对NCI-H446有杀伤作用,杀伤作用与病毒剂量和作用时间呈正相关.13417株对HFL-1也有杀伤作用,但比同等条件下对NCI-H446的杀伤作用弱.D417株感染NCI-H446后光镜下可见细胞病变及细胞融合现象,电镜下可见细胞呈凋亡形态. 结论 NDV D417株对小细胞肺癌细胞有较强的杀伤作用,对正常细胞影响较小.
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| 关 键 词： | 新城疫病毒 癌 小细胞 体外研究 |
Oncolytic effect of Newcastle disease virus strain D417 On small cell lung cancer in vitro |
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| SONG De-zhi,YANG Jian-lin,JIANG Yan-hua,HUANG Chuan,LAI Zhen-bing,MAO Nai-quan,FAN Xiao-hui. Oncolytic effect of Newcastle disease virus strain D417 On small cell lung cancer in vitro[J]. International Journal of Biologicals, 2008, 31(3): 97-100. DOI: 10.3760/cma.j.issn.1673-4211.2008.03.001 |
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| Authors: | SONG De-zhi YANG Jian-lin JIANG Yan-hua HUANG Chuan LAI Zhen-bing MAO Nai-quan FAN Xiao-hui |
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| Abstract: | Objective To study the cytolytic effect of D417 strain of Newcastle disease virus (NDV)on small cell lung cancer cell line NCI-H446 and normal cell line HFL-1. Methods Death rates of NCI-H446 and HFL-1 cells were measured through methyl thiazolyl tetrazolium eolorimetric as-say after the cells were infected by NDV strain D417 in vitro.Pathological changes of NCI-H446 cells were observed under light and electron microscopes. Results NDV strain IMl7 killed NCI-H446 cells.and the eell death rate was positively correlated with viral dose and incubation time.Strain D417 also kill-ed HFL-l cells,but the death rate of infected HFL-1 cells was lower than that of NCI-H446 cells under the same condition.After strain D417 infection.the cytopathogenic effect and fusion of NCI-H446 cells were found under the light microscope,apoptosises were found by electron microscopy. Conclusions NDV strain D417 has the ability to kill small cell lung cancer cells and a relatively less influence on nor-mal cells. |
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| Keywords: | Newcastle disease virus Carcinoma,small cell In vitro |
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