| 骨髓间充质干细胞骨向诱导分化过程中破骨细胞分化因子和细胞间粘附分子-1的表达变化 |
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| 引用本文： | 王军,赵志河,罗颂椒,樊瑜波. 骨髓间充质干细胞骨向诱导分化过程中破骨细胞分化因子和细胞间粘附分子-1的表达变化[J]. 华西口腔医学杂志, 2005, 23(3): 240-243 |
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| 作者姓名： | 王军 赵志河 罗颂椒 樊瑜波 |
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| 作者单位： | 1.口腔生物医学工程教育部重点实验室;2.四川大学生物力学工程实验室,四川 成都610041 |
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| 基金项目： | 国家自然科学基金资助项目(10372066,10402027) |
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| 摘 要： | 目的 观察破骨细胞分化因子(ODF)和细胞间粘附分子-1(ICAM-1)在不同分化状态成骨细胞的表达变化,探讨正畸牙移动过程中成骨细胞对破骨细胞分化成熟的诱导机制。方法 分离培养大鼠骨髓间充质干细胞,成骨定向诱导后获得不同分化状态的成骨细胞,RT-PCR检测不同分化状态下的成骨细胞ODF和ICAM-1的表达变化。结果 成骨细胞在分化成熟过程中,ICAM-1 mRNA表达水平逐渐升高;ODF mRNA则在诱导后6 d开始表达,并维持在一较稳定的水平。结论 不同分化状态的成骨细胞对破骨细胞诱导分化的能力有所差异,相对成熟的成骨细胞的诱导能力可能更强。
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| 关 键 词： | 骨髓间充质干细胞 成骨细胞 破骨细胞分化因子 细胞间粘附分子-1 |
| 文章编号： | 1000-1182(2005)03-0240-04 |
| 收稿时间： | 2005-06-25 |
| 修稿时间： | 2005-01-12 |
Expression of Osteoclast Differentiation Factor and Intercellular Adhesion Molecule-1 of Bone Marrow Mesenchymal Stem Cells Enhanced with Osteogenic Differentiation |
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| WANG Jun,ZHAO Zhi-he,LUO Song-jiao,FAN Yu-bo. Expression of Osteoclast Differentiation Factor and Intercellular Adhesion Molecule-1 of Bone Marrow Mesenchymal Stem Cells Enhanced with Osteogenic Differentiation[J]. West China journal of stomatology, 2005, 23(3): 240-243 |
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| Authors: | WANG Jun ZHAO Zhi-he LUO Song-jiao FAN Yu-bo |
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| Affiliation: | 1.Key. Laboratory ofOral Biomedical EngineeringMinistry ofEducation,Sichuan University,Chengdu610041,China; 2.Laboratory of Biomechanical Engineering,Sichuan University,Chengdu610041,China |
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| Abstract: | Objective To investgate whether the expression of osteoclast differentiation factor(ODF), intercellular adhesion molecule_1(ICAM-1) depended on the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells(BMSC). Methods BMSC (4 passage) were selected for osteogenic differentiation by treated with osteogenic supplements(OS). Cells were harvested by day 0, 3, 6, 9, 12, 18 respectively after osteogenic inducement. In each experiment, control and OS-treated cells were processed in parallel. ODF and ICAM-1 mRNA were analyzed by semiquantitave RT-PCR assay.Results Expression of ODF was enhanced with osteogenic differentiation guadully. whereas, expression of ICAM-1 was activated at OS-treated day 6, then keeping at a stable level. Conclusion This study indicated that BMSC undergoing osteogenic inducement was an ideal model for studying the differentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts from stem cells to osteocytes, BMSC or osteoprogenitor react somewhat differently from osteoblasts, suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have been improved with osteogenic culture. |
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| Keywords: | rat bone marrow mesenchymal stem cells osteoblasts osteoclast differentiation factor intercellular adhesion molecule_1 |
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