| 23例非小细胞肺癌患者肺癌组织和血浆中EGFR外显子21的基因突变检测 |
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| 引用本文： | 惠福海,童向东,孙玉坤,孙丹,王述民,程刚,赵庆春. 23例非小细胞肺癌患者肺癌组织和血浆中EGFR外显子21的基因突变检测[J]. 沈阳药科大学学报, 2010, 27(11): 928-932 |
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| 作者姓名： | 惠福海 童向东 孙玉坤 孙丹 王述民 程刚 赵庆春 |
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| 作者单位： | （1.沈阳军区总医院 药剂科,辽宁 沈阳 110840;2.沈阳药科大学 药学院,辽宁 沈阳 110016;3.大连医科大学 药学院,辽宁 大连 116044） |
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| 摘 要： | 目的证明可以从非小细胞肺癌患者血浆中检测到表皮生长因子受体(epidermal growth factorreceptor,EGFR)突变,且与肿瘤组织的突变情况相一致,建立一种从外周血液中快速、经济、准确检测EGFR外显子21基因突变的方法。方法同时取得23例非小细胞肺癌患者的肺癌组织和血浆,采用突变富集PCR方法检测外周血液中EGFR外显子21的基因突变,并将检测结果与肺癌组织的检测结果进行对比。结果 23例非小细胞肺癌患者的肺癌组织中共检测到8例EGFR外显子21突变,其中7例突变可以从患者的外周血液中检测到。结论可以从非小细胞肺癌患者外周血液中检测到EGFR突变,通过本方法,可以从外周血液中检测EGFR外显子21基因突变。
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| 关 键 词： | 表皮生长因子受体 非小细胞肺癌 基因突变 血浆 |
| 收稿时间： | 2010-03-08 |
Detection of epidermal growth factor receptor exon 21 mutations in tissue and plasma in 23 cases of non-small cell lung cancer |
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| HUI Fu-hai,TONG Xiang-dong,SUN Yu-kun,SUN Dan,WANG Shu-min,CHENG Gang,ZHAO Qing-chun. Detection of epidermal growth factor receptor exon 21 mutations in tissue and plasma in 23 cases of non-small cell lung cancer[J]. Journal of Shenyang Pharmaceutical University, 2010, 27(11): 928-932 |
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| Authors: | HUI Fu-hai TONG Xiang-dong SUN Yu-kun SUN Dan WANG Shu-min CHENG Gang ZHAO Qing-chun |
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| Affiliation: | （1.The Genearl Hospitl of Shengyang Military Region, Shenyang 110016, China; 2. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. School of Pharmacy, Dalian Medical University, Dalian 116044, China） |
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| Abstract: | To develop a novel method for detecting the exon 21 mutations of epidermal growth factor receptor(EGFR) from the non-small cell lung cancer patients’ plasma. Methods The tissue samples and plasma of 32 non-small cell lung cancer patients were collected simultaneously, the results of detecting the exon 21 mutations of epidermal growth factor receptor(EGFR)from plasma compare with that from the tissue sample by the method of mutant-enriched PCR. Results Eight cases of the exon 21 mutation of epidermal growth factor receptor(EGFR) from 23 were detected from tissue samples, 7 cases of the exon 21 mutation of epidermal growth factor receptor(EGFR) can be detected from plasma.Conclusion The EGFR mutation can be detected in plasma from patients with non-small lung cancer,the exon 21 mutations of EGFR can be detected by this method. |
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| Keywords: | EGFR(epidermal growth factor receptor) non-small cell lung cancer mutation plasma |
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