Human T cell responses to recombinant mite antigens of Dermatophagoides farinae |
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| Authors: | S FUJII K ONO S SHIGETA T JYO U YAMASHITA |
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| Affiliation: | Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Hiroshima, Japan;*Third Department of Internal Medicine, Hiroshima Prefectural Hospital, Hiroshima, Japan;†Department of Immunology and Parasitology, School of Medicine, Hiroshima University, Hiroshima, Japan |
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| Abstract: | We studied T cell responses to four glutathione S transferase (GST)-fused mite antigens prepared in our laboratory using peripheral blood lymphocytes from mite-sensitive patients with bronchial asthma. Of the four recombinant antigens, purified GST-Mag3 had the strongest ability to cause patients' lymphocytes to proliferate, and its potency was almost comparable to that of crude mite bodies (Dfb) and faeces (Dff) antigens and a purified major antigen, Der f 2. The responder lymphocytes were mainly T cells, because the proliferative response was depleted by the treatment of lymphocytes with anti-CD3 antibody and complement, but not with anti-CD20 antibody and complement. The responsiveness of lymphocytes to GST-Mag3 correlated with that to Der f 2, but GST-Mag3 displayed slightly higher activity to stimulate lymphocytes than Der f 2. Simultaneously, the levels of Dff- and GST-Mag3-specific IgE antibodies correlated with the responsiveness of lymphocytes to GST-Mag3. These results suggest that Mag3 is a new valuable antigen for the response of T cell proliferation in mite-sensitive patients. |
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| Keywords: | Dermatophagoides farinae recombinant antigen GST-fused protein T cell response |
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