Partial recovery of senescence and differentiation disturbances in CD8+ T cell effector‐memory cells in HIV‐1 infection after initiation of anti‐retroviral treatment |
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| Authors: | J. M. Eberhard F. Ahmad H. S. Hong N. Bhatnagar P. Keudel J. Schulze zur Wiesch R. E. Schmidt D. Meyer‐Olson |
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| Affiliation: | 1. Klinik für Immunologie und Rheumatologie, Medizinische Hochschule Hannover, Hannover, Germany;2. Zentrum für Innere Medizin, Universit?tsklinikum Hamburg‐Eppendorf, Hamburg, Germany;3. Division of Immunology, New England Primate Research Center, Harvard Medical School, Southborough, MA, USA;4. Unit of Cytokines and Inflammation, Institute Pasteur, Paris, France;5. DZIF German Center for Infection‐Partner Sites Hamburg, Hannover, Germany |
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| Abstract: | Immune senescence as well as disturbed CD8+ T cell differentiation are a hallmark of chronic HIV infection. Here, we investigated to what extent immune senescence is reversible after initiation of anti‐retroviral treatment (ART). Peripheral blood mononuclear cells (PBMCs) from a cohort of HIV patients with different disease courses, including untreated viral controllers (n = 10), viral non‐controllers (n = 16) and patients on ART (n = 20), were analysed and compared to uninfected controls (n = 25) by flow cytometry on bulk and HIV‐specific major histocompatibility complex (MHC) class I tetramer+ CD8+ T cells for expression of the memory markers CCR7 and CD45RO, as well as the senescence marker CD57 and the differentiation and survival marker CD127. Furthermore, a subset of patients was analysed longitudinally before and after initiation of ART. Frequencies of CD57+CD8+ T cells decreased after initiation of ART in central memory (Tcm) but not in effector memory T cell populations (TemRO and TemRA). The frequency of CD127+CD8+ cells increased in Tcm and TemRO. We observed a reduction of CD127– T cells in Tcm, TemRO and partially in TemRA subsets after initiation of ART. Importantly, HIV‐specific CD8+ TemRO cells predominantly displayed a CD127–CD57+ phenotype in untreated HIV‐patients, whereas the CD127+CD57– phenotype was under‐represented in these patients. The frequency of the CD127+CD57–CD8+ T cell subpopulation correlated strongly with absolute CD4+ counts in HIV‐infected patients before and after initiation of ART. These findings can be interpreted as a phenotypical correlate of CD8+ memory T cell differentiation and the premature ‘ageing’ of the immune system, which was even observed in successfully virally suppressed HIV patients. |
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| Keywords: | AIDS cytotoxic T cells T cells viral |
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