Lividomycin Resistance in Staphylococci by Enzymatic Phosphorylation |
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| Authors: | Fujio Kobayashi Tomoyuki Koshi Junji Eda Yoko Yoshimura Susumu Mitsuhashi |
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| Affiliation: | .Tokyo Research Laboratories, Kowa Co., Ltd., Higashimurayama, Tokyo, Japan;.Department of Microbiology, School of Medicine, Gunma University, Maebashi, Japan |
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| Abstract: | Enzymatic inactivation of lividomycin (LV) was attempted with nine clinical isolates of staphylococci including LV-susceptible and -resistant strains. LV inactivation and the incorporation into LV of (32)P from gamma-(32)P-adenosine triphosphate were demonstrated in the presence of cell-free extracts from LV-resistant strains but not from LV-susceptible ones. The enzyme was purified approximately 82-fold from a resistant Staphylococcus aureus strain by means of ammonium sulfate fractionation and column chromatography. Some properties of the partially purified LV-phosphorylating enzyme were quite similar to those of an enzyme from Escherichia coli carrying an R factor conferring LV resistance, and the phosphorylated product of the drug was also found to be identical with that produced by E. coli carrying an R factor, i.e., 5'-phosphoryl-LV. |
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