| 磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究 |
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| 引用本文： | 磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究. 磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究[J]. 首都医科大学学报, 2015, 36(5): 729-733. DOI: 10.3969/j.issn.1006-7795.2015.05.013 |
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| 作者姓名： | 磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究 |
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| 作者单位： | 首都医科大学基础医学院细胞生物学系肝脏保护与再生调节北京市重点实验室, 北京 100069 |
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| 基金项目： | 国家自然科学基金(81430013), 北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20150502)。 |
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| 摘 要： | 目的 本课题探究了磷酸鞘胺醇(sphingosine 1-phosphate, S1P)对小鼠单核巨噬细胞J774A.1表达炎性细胞因子的调节机制。方法 应用蛋白印迹实验检测小鼠单核巨噬细胞J774A.1是否表达S1P受体,进一步应用实时荧光定量聚合酶链反应检测小鼠单核巨噬细胞J774A.1在S1P刺激下是否表达炎性细胞因子,最后应用药理学工具干预S1P受体(S1P receptor, S1PRs)信号系统研究小鼠单核巨噬细胞J774A.1表达炎性细胞因子的调节机制。结果 S1PR2拮抗剂(JTE-013)、S1PR3拮抗剂(CAY-10444)可抑制小鼠单核巨噬细胞系J774A.1炎性细胞因子的表达;而S1PR1拮抗剂(W146)对小鼠单核巨噬细胞炎性细胞因子的表达并无影响。结论 磷酸鞘胺醇通过S1PR2和S1PR3上调小鼠单核巨噬细胞J774A.1炎性细胞因子的表达。
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| 关 键 词： | 单核巨噬细胞 磷酸鞘胺醇 炎性反应因子 |
| 收稿时间： | 2015-04-21 |
Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1 |
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| Zhang Yuanyuan,Li Weiyang,Yang Lin,Li Liying. Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1[J]. Journal of Capital Medical University, 2015, 36(5): 729-733. DOI: 10.3969/j.issn.1006-7795.2015.05.013 |
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| Authors: | Zhang Yuanyuan Li Weiyang Yang Lin Li Liying |
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| Affiliation: | Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China |
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| Abstract: | Objective The aim of study is to explore the role of sphingosine 1-phosphate (S1P) signaling on murine monocyte/macrophage inflammatory cytokine expression. Methods Western blotting was employed to test whether S1P receptors (S1PRs) expression in murine monocyte/macrophages. Then we detected whether S1P-mediated expression of inflammatory cytokines in murine monocyte/macrophage J774A.1 by real-time PCR. We studied regulatory mechanisms of expression of inflammatory cytokines in J774A.1 cells by intervention of S1P/S1PRs signaling with pharmacology tools. Results S1PR2 antagonist JTE-013, S1PR3 antagonist CAY-10444 treatment reduced expression of inflammatory cytokines in J774A.1 cells. S1PR1 antagonist W146 has no effects on the expression of inflammatory cytokines in J774A.1 cells. Conclusion S1P up-regulates expression of inflammatory cytokines in J774A.1 cells mediated by S1PR2 and S1PR3. |
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| Keywords: | monocyte/macrophage sphingosine 1-phosphate inflammatory cytokine |
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