| 尿液中miRNA-24-3p联合miRNA-222-3p检测在前列腺癌中的诊断及临床意义 |
| |
| 引用本文： | 任超1,2. 尿液中miRNA-24-3p联合miRNA-222-3p检测在前列腺癌中的诊断及临床意义[J]. 天津医科大学学报, 2022, 0(2): 181-185 |
| |
| 作者姓名： | 任超1 2 |
| |
| 作者单位： | （1.天津医科大学人民医院临床学院，天津300121；2.天津市滨海新区大港医院检验科，天津300270） |
| |
| 摘 要： | 目的:建立尿液miRNA检测板，作为诊断前列腺癌（PC）的无创性生物标志物。方法:采用miRNAs芯片分析对照1组（6名）及PC1组（18例）中miRNAs表达，并进一步利用qRT-PCR对20名对照2组及59例PC2组（GS2 6组22例，GS2 7组19例，GS28组18例）尿液、血清标本进行差异基因的进一步验证。应用受试者工作特征（ROC）曲线分析组合诊断模型的准确真实性。结果:获得在PC1组中20个miRNAs差异表达谱，与对照1组相比，miRNA-24-3p及miRNA-222-3p显著表达下调（T=5.79、4.59，均P＜0.05），用于PC诊断检测。在扩大样本量的PC患者尿液及血清样本中，根据GS分组的GS26、GS27及GS28组中，与对照2组相比，miRNA-24-3p联合miRNA-222-3p表达水平也都显著下调（t=3.89，P＜0.05），ROC分析联合诊断模型具有较高的准确度（曲线下面积为0.93，OR=1.37，95% CI:0.92~1.76，P＝0.02）。结论:在尿液、血清样本中建立了miRNA-24-3p联合miRNA-222-3p组合模型，可作为诊断PC的无创性生物标志物。
|
| 关 键 词： | miRNAs 前列腺癌 生物标志物 qRT-PCR |
Diagnosis and clinical significance of miR-24b-3p combined with miR-222-3p in urinary in prostate cancer |
| |
| REN Chao1,' target="_blank" rel="external">2. Diagnosis and clinical significance of miR-24b-3p combined with miR-222-3p in urinary in prostate cancer[J]. Journal of Tianjin Medical University, 2022, 0(2): 181-185 |
| |
| Authors: | REN Chao1,' target=" _blank" rel=" external" >2 |
| |
| Affiliation: | （1. Tianjin Union Medical Center，Tianjin Medical University，Tianjin 300121，China； 2. Department of Clinical Laboratory，Dagang Hospital of Tianjin Binhai New Area，Tianjin 300270，China） |
| |
| Abstract: | Objective: To establish a urine miRNA detection panel as a non-invasive biomarker helping diagnosing prostate cancer （PC）.Methods: A total of 18 PC1 group patients and 6 normal control group 1 urinary samples were analyzed through a miRNA microarray process，and further using quantitative real-time polymerase chain reaction，differentially expressed miRNAs were detected in urinary and serum of the PC2 patients（n=59），including 22 GS2 6，19 GS2 7 and 18 samples of GS2 8，20 normal samples as the control group 2.The accuracy and authenticity of the combined diagnostic model should be analyzed by the receiver operating characteristic（ROC）curve. Results: The differential expression profiles of 20 miRNAs in PC1 group were identified，including miR-24b-3p and miR-222-3p for PC detection，compared with control group1（T=5.79，4.59，all P＜0.05）. Moreover，in the urine and serum samples of PC samples with expanded samples，the expression of miR-24b-3p combined with miR-222-3p in GS6，GS7 and GS8 groups grouped according to GS score were significantly decreased than those in the control group 2（t=3.89，P<0.05）. The combined diagnostic model of ROC analysis has high accuracy（area under curve=0.93，OR=1.37，95% CI:0.92-1.76，P＝0.02）. Conclusion: A combined model of miR-24-3p and miR-222-3p in urinary and serum samples are established，which can be acted as a non-invasive biomarker helping diagnosing PC. |
| |
| Keywords: | miRNAs prostate cancer diagnostic biomarker qRT-PCR |
|
| 点击此处可从《天津医科大学学报》浏览原始摘要信息 |
|
点击此处可从《天津医科大学学报》下载免费的PDF全文 |
