Circulating MicroRNA-21 Expression as a Novel Serum Biomarker for Oral Sub-Mucous Fibrosis and Oral Squamous Cell Carcinoma |
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| Authors: | Pooja Singh1Anand Narain Srivastava1Rolee Sharma2Saboor Mateen3Bharat Shukla4Alok Singh1Siddhartha Chandel5 |
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| Affiliation: | 1Department of Pathology, Era’s Lucknow Medical College and Hospital, Lucknow.2Department of Biosciences, Integral University, Lucknow.3Department of Medicine, Era’s Lucknow Medical College and Hospital, Lucknow.4Oral and Maxillofacial Department, Sardar Patel Post Graduate Institute of Dental and Medical Sciences, Lucknow.5Department of Dentistry,Era's Lucknow Medical College and Hospital, Lucknow. |
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| Abstract: | Background: Circulating miRNAs (miRs) in the biofluids such as serum and plasma act as potential biomarkersfor early diagnosis, treatment and prognosis. In the present study, an attempt made to see the expression of miR-21in serum of 20 cases of Oral sub-mucous fibrosis (OSMF), 20 cases of Oral squamous cell carcinoma and 40 healthyvolunteers. The expression of miR-21 was evaluated in relation to different demographical and clinicopathologicalfeatures such as sex, tobacco, pan-masala, alcohol, smoking and clinical staging respectively with an aim to identifycorrelation with oral pre-cancer and cancer stages. Materials and Methods: The relative expression level of miR-21was determined by quantitative real-time RT-PCR (qRT-PCR) in the sera of 20 OSCC, 20 OSMF patients and 40healthy subjects as a control. Association between expression of miR-21 and OSCC clinical stages and demographicalparameters such as sex, pan-masala, tobacco, smoking, alcohol have also been analyzed in detail. Results: The resultsobtained by t-test revealed significant increase in the expression level of miR-21 in OSCC as compared to OSMF. Thestudy also revealed the positive correlation between higher miR-21 expression and pan-masala chewers as shown byt-test. The statistical test, ANOVA has also indicated a positive correlation between up-regulation of miR-21 in theclinical stages of the OSCC. Conclusion: The results of present study indicated up-regulation of circulating miR-21 inserum of OSCC as compared to OSMF (p=0.001), this study also elucidated the positive correlation between miR-21expression in OSCC/OSMF patients, only one demographical parameter (Pan-masala) and negative correlation forother parameters such as sex, tobacco, smoking, alcohol etc. Other findings suggested a significant increase (p=0.000)in the expression of miR-21 in clinical staging (I-IV) of oral cancer. More studies are needed to validate it as potentialdiagnostic and prognostic biomarker for OSMF and OSCC for better management. |
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| Keywords: | Circulating miRNA Non-invassive biomarker miR21 |
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