| 长链非编码RNA-ROR对结直肠癌SW620细胞增殖的影响 |
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| 引用本文： | 施炜,胡世丰,雷春,吴伙. 长链非编码RNA-ROR对结直肠癌SW620细胞增殖的影响[J]. 蚌埠医学院学报, 2019, 44(10): 1297-1300. DOI: 10.13898/j.cnki.issn.1000-2200.2019.10.001 |
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| 作者姓名： | 施炜 胡世丰 雷春 吴伙 |
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| 作者单位： | 铜陵职业技术学院 医学系基础形态教研室,安徽 铜陵,244061;安徽省铜陵市人民医院 普外科, 244000;安徽医科大学第一附属医院(高新院区) 肝胆外科,安徽 合肥,230088 |
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| 基金项目： | 安徽省教育厅高校自然科学研究重点项目KJ2017A635 |
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| 摘 要： | 目的了解长链非编码RNA-ROR（LncRNA-ROR）在结直肠癌SW620细胞增殖中的作用。方法选择40例结直肠癌组织及相应正常组织（距肿瘤切缘5cm以外），首先采用qRT-PCR技术检测LncRNA-ROR在结直肠癌组织中的表达情况，并分析其表达与病人临床病理因素的相关性。构建慢病毒载体LV5-ROR、LV5-Vector转染到结直肠癌细胞SW620中，验证LncRNA-ROR的表达，分析LncRNA-ROR过表达对结直肠癌细胞CCK-8功能及SW620平板克隆形成的影响。结果与正常组织比较，LncRNA-ROR在结直肠癌组织中低表达（P < 0.01），其表达水平与TNM分期具有相关性（P < 0.05）。经转染筛选后，qRT-PCR检测证实SW620中的LncRNA-ROR表达增加，其高表达导致SW620增殖能力的下降（P < 0.01）。结论LncRNA-ROR的过表达抑制了结直肠癌细胞SW620的增殖。
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| 关 键 词： | 结直肠肿瘤 LncRNA-ROR SW620细胞 增殖 |
| 收稿时间： | 2019-06-18 |
Effect of LncRNA-ROR on the proliferation of colorectal cancer SW620 cells |
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| Affiliation: | 1.Department of Medical Morphology, Department of Medicine, Tongling Vocational and Technical College, Tongling Anhui 2440612.Department of General Surgery, Tongling People's Hospital, Tongling Anhui 2440003.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University(High-tech Institute), Hefei Anhui 230088, China |
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| Abstract: | ObjectiveTo investigate the effects of long non-coding RNA-ROR(LncRNA-ROR) on the proliferation of colorectal cancer SW620 cells.MethodsThe expression level of LncRNA-ROR in colorectal cancer tissue was detected using qRT-PCR, and the relationship between the expression level of LncRNA-ROR and clinicopathological data was analyzed.The lentiviral vector LV5-ROR was constructed, and transfected into the colorectal cancer SW620 cells and verified.The effects of LncRNA-ROR overexpression on the function and colony formation of SW620 cells were analyzed.ResultsCompared with the normal tissue, the expression level of LncRNA-ROR was low in colorectal cancer tissue(P < 0.01), and its expression level was closely related to TNM stage(P < 0.05).After transfection, the expression level of LncRNA-ROR increasing in SW620 cells was identified by qRT-PCR, and its high expression led to the decreasing of proliferation function of SW620 cells(P < 0.01).ConclusionsThe overexpression of LncRNA-ROR can inhibit the proliferation of SW620 cells. |
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