| 东方田鼠非酒精性脂肪肝模型的建立 |
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| 引用本文： | 杨玉琴,冯洁,柏熊,沈志敏,刘雄伟,高捷,谢建云,胡建华,高诚. 东方田鼠非酒精性脂肪肝模型的建立[J]. 实验动物与比较医学, 2013, 21(2) |
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| 作者姓名： | 杨玉琴 冯洁 柏熊 沈志敏 刘雄伟 高捷 谢建云 胡建华 高诚 |
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| 作者单位： | 上海市公共卫生临床中心,上海市实验动物质量监督检验站,上海实验动物研究中心,,上海西普尔-必凯实验动物有限公司,上海实验动物研究中心,上海市实验动物质量监督检验站,上海实验动物研究中心,上海市实验动物质量监督检验站,上海实验动物研究中心,上海西普尔-必凯实验动物有限公司,上海实验动物研究中心,上海市实验动物质量监督检验站,上海实验动物研究中心,上海市实验动物质量监督检验站,上海实验动物研究中心,上海市实验动物质量监督检验站,上海实验动物研究中心 |
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| 基金项目： | 上海市科学基因资助项目（071409001,11140902801） |
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| 摘 要： | [摘要] 目的：建立东方田鼠非酒精性脂肪肝模型,并观察测定其肝指数、病理、血清生化指标的动态变化。方法：选取6周龄湖南洞庭湖种群雄性东方田鼠70只,随机分为2组,模型组饲喂高脂肪料,对照组饲喂高纤维料,分别于第2、4、6、8、12周处死,称量体重及肝重,计算肝指数,采血检测东方田鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、r-谷氨酰转移酶（r-GT）、胆碱酯酶（CHE）、甘油三酯（TG）、总胆固醇（TC）、游离脂肪酸（FFA）、葡萄糖（GLU）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）,并取肝脏HE染色后做病理学观察。结果：与对照组相比,模型组6周时肝脏出现了典型的脂肪肝特征,肝重和肝指数都明显升高（P﹤0.05）,血清ALT、AST、r-GT、CHE、TC、FFA、GLU和LDL都明显升高（P﹤0.05）,HDL和TG均明显降低（P﹤0.05）。镜检观察到模型组田鼠肝细胞逐渐呈现弥漫性脂肪变性,6周时大范围出现脂滴,8周时肝内出现弥漫性大脂肪滴,12周后出现炎细胞的浸润。结论：采用高脂饲料成功诱发东方田鼠非酒精性脂肪肝,可为研究非酒精性脂肪肝的发病机制和药物干预提供新的模型。
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| 关 键 词： | 东方田鼠 非酒精性脂肪肝 |
| 收稿时间： | 2012-10-25 |
| 修稿时间： | 2012-11-16 |
The Establishment of Non-alcoholic Fat Liver Animal Model of Microtus fortis |
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| Abstract: | Objective: To establish the Non-alcoholic Fat Liver Animal Model of Microtus fortis and to observe the dynamic variation of relevant index. Methods: 70 6-week-old male Microtus fortis were randomly divided into two groups. The voles of model group were fed on the high-fat ingredients and the voles of control group were fed on the high-fiber diet. Voles of each group were sacrificed at 2, 4, 6, 8, 12 week. The body weight and liver weight were measured; serum values of ALT, AST, ALP, r-GT, CHE, TG, TC, FFA, GLU, HDL and LDL were calculated by biochemistry analyzer. The histological changes of livers were observed with light microscopy. Results: Voles fed the high-fat diet developed panlobular macrovesicular steatosis, whereas those fed the high-fiber diet had normal livers. Compared with the control group, liver weight and liver index of model group were significantly increased, serum ALT, AST, r-GT, CHE, TC, FFA, GLU and LDL were significantly high; HDL and TG were significantly low. Conclusions: A Non-alcoholic fatty liver model of Microtus fortis was successfully established by fed high-fat diet for 6 weeks, which could provide a useful tool to elucidate the pathogenesis and the treatment of the NAFL. |
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| Keywords: | Microtus fortis Non-alcoholic fatty liver disease |
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