| 三氧化二砷对人肺腺癌A549/R细胞多药耐药相关蛋白表达的影响 |
| |
| 引用本文： | 成静,冯觉平,王亚萍,李敏,孔庆志. 三氧化二砷对人肺腺癌A549/R细胞多药耐药相关蛋白表达的影响[J]. 医药导报, 2007, 26(5): 457-460 |
| |
| 作者姓名： | 成静 冯觉平 王亚萍 李敏 孔庆志 |
| |
| 作者单位： | 1. 武汉大学中南医院肿瘤科,430071;武汉市普爱医院肿瘤科,430030
2. 武汉市普爱医院肿瘤科,430030 |
| |
| 基金项目： | 甘肃省兰州市科技局资助项目 |
| |
| 摘 要： | 目的探讨三氧化二砷(As2O3)对人肺腺癌A549/R细胞耐药性的逆转作用及对多药耐药相关蛋白（MRP）表达的影响。方法以荧光分光光度计测定细胞内药物浓度的改变，采用半定量逆转录聚合酶联反应(RT PCR) 技术检测As2O3处理后A549/R MRP基因表达的变化。结果As2O3的非细胞毒性剂量可增加A549/R细胞内多柔比星(ADM)浓度，降低其IC50。A549/R细胞中MRP呈过表达状态，不同浓度的As2O3处理A549/R后MRP表达水平明显降低。结论As2O3可部分逆转A549/R细胞对ADM的耐药性，其逆转机制与改变MRP基因表达有关。
|
| 关 键 词： | 三氧化二砷 人肺腺癌A549/R细胞 多药耐药 多药耐药相关蛋白 |
| 文章编号： | 1004-0781（2007）05-0457-04 |
| 收稿时间： | 2007-02-01 |
| 修稿时间： | 2007-02-01 |
Effect of Arsenic Tioxide on MRP Expression in A549/R Cell Line of Human Lung Cancer |
| |
| CHENG Jing,FENG Jue-ping,WANG Ya-ping,LI Min,KONG Qing-zhi. Effect of Arsenic Tioxide on MRP Expression in A549/R Cell Line of Human Lung Cancer[J]. Herald of Medicine, 2007, 26(5): 457-460 |
| |
| Authors: | CHENG Jing FENG Jue-ping WANG Ya-ping LI Min KONG Qing-zhi |
| |
| Abstract: | Objective To investigate the effect of arsenic trioxide(As2O3) on the reversal role of drug resistance and the expression of MRP in human lung cancer cell line A549/R. Methods The comcentration of intracellular ADM was detected by fluorescence spectrofluorometry.The expression of MRP gene was assessed in As2O3-treated A549/R cells by semiq-uantitative RT-PCR. Results As2O3 in noncytotoxic dose could significantly increase the intracellular accumulation of ADM in A549/R cell line and derease the medium inhibition concentration(IC50).The overexpression of MRP mRNA was observed in A549/R cell line and this was significantly suppressed in As2O3-treated A549/R cells. Conclusion As2O3 can partically reverse the resistance to ADM in A549/R cell line and this effect may be related to the variation of MRP gene expression. |
| |
| Keywords: | Arsenic trioxide Human lung adenocarcinoma A549/R cell Multidrug resistance MRP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《医药导报》浏览原始摘要信息 |
|
点击此处可从《医药导报》下载全文 |
