| 大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用 |
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| 引用本文： | 罗海峰,杜渐,孟辉,巩鹏,牟国煜,谭广,王洪江. 大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用[J]. 大连医科大学学报, 2013, 0(6): 526-529 |
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| 作者姓名： | 罗海峰 杜渐 孟辉 巩鹏 牟国煜 谭广 王洪江 |
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| 作者单位： | 大连医科大学附属第一医院肝胆外科,辽宁大连116011 |
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| 基金项目： | 大连市科委基金资助项目（2011E15SF114） |
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| 摘 要： | 目的：观察大豆异黄酮载药纳米颗粒对肝纤维化大鼠的抗过氧化作用。方法采用四氯化碳灌胃法建立肝纤维化大鼠模型，随机分成对照组、肝纤维化组、空白纳米颗粒组（ SPIO组）、大豆异黄酮组（ SI组）、载药纳米颗粒组（ SI-SPIO组），每组24只。对照组给予生理盐水尾静脉注射，其他各组除四氯化碳灌胃外，分别给予生理盐水尾静脉注射、SPIO、大豆异黄酮和SI-SPIO载药纳米颗粒尾静脉注射治疗，观察对照组和治疗组之间肝脏生化指标、过氧化损伤指标和肝脏组织学变化。结果8周后对各组大鼠的肝脏生化指标和肝纤维化程度进行评估，SI组和SI-SPIO组明显优于肝纤维化组和SPIO组；过氧化指标检测，与对照组相比，肝纤维化组和SPIO组MDA明显升高（2．45±0．77，2．69±1．01，P＜0．05），GSH -Px 和SOD明显降低（300．74±16．25，299．18±15．57和409．77±20．46，431．56±19．25，P＜0．05）；与肝纤维化组相比，SI组和SI-SPIO组MDA明显降低（0．85±0．12，0．90±0．02，P＜0．05），GSH-Px和SOD明显升高（340．25±11．85，366．45±14．83和467．27±16．11，485．75±22．54，P＜0．05）。 SI-SPIO组过氧化指标较SI组改善更为明显（P＜0．05）。结论大豆异黄酮具有抗氧化作用，能够抑制大鼠肝纤维化形成，载药纳米有助于药效的发挥。
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| 关 键 词： | 大豆异黄酮 肝纤维化 过氧化损伤 |
Anti-peroxidative effects of SI-SPIO nanoparticles in liver fibrotic rats |
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| LUO Hai-feng,DU Jian,MENG Hui,GONG Peng,MU Guo-yu,TAN Guang,WANG Hong-jiang. Anti-peroxidative effects of SI-SPIO nanoparticles in liver fibrotic rats[J]. Journal of Dalian Medical University, 2013, 0(6): 526-529 |
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| Authors: | LUO Hai-feng DU Jian MENG Hui GONG Peng MU Guo-yu TAN Guang WANG Hong-jiang |
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| Affiliation: | (Hepatobiliary Surgical Department, the First Affdiated Hospital of Dalian Medical University, Dalian 116011, China) |
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| Abstract: | Objective To investigate the anti -peroxidative effects of SPIO nanoparticles as a drug delivery system when combined with soybean isoflavones （ SI-SPIO ） .Methods The liver fibrosis models of rats were established by CCL 4 ga-vage.120 rats were random grouped as control , fibrosis, SPIO tail vein injection, isoflavone treatment and SI -SPIO treat-ment group .Normal saline injections were given in control group , CCL4 gavage were given in other groups , as well as NS injection, SPIO, SI and SI-SPIO injections to 24 rats of each group, respectively.Biochemical and histological indicators were analyzed .Peroxidative indicators were detected as well .Results Liver fibrosis was significantly improved in SI and SI-SPIO groups vs .control both in biochemical and histological aspects .Peroxcidative indicators such as MDA increased in liver fibrotic and SPIO groups （2.45 ±0.77, 2.69 ±1.01, P〈0.05）, GSH -Px and SOD decreased （300.74 ± 16.25, 299.18 ±15.57 and 409.77 ±20.46, 431.56 ±19.25, P〈0.05）, SI administration reduced MDA （0.85 ± 0.12,0.90 ±0.02, P〈0.05）, increased GSH -Px and SOD in SI and SI -SPIO groups（340.25 ±11.85, 366.45 ± 14.83 and 467.27 ±16.11, 485.75 ±22.54, P〈0.05）.SI-SPIO group presents much more efficiency comparing with SI group （P〈0.05）.Conclusion SI has anti-peroxidative effect and inhibits liver fibrosis in rats .SPIO nanoparticles make the effects more efficient as a drug delivery system . |
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| Keywords: | soybean isoflavones liver fibrosis peroxidative damage |
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