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| 消除水体基体干扰水中痕量丙烯酰胺HPLC-MS/MS检测 | |
| 引用本文： | 张琦, 马金波. 消除水体基体干扰水中痕量丙烯酰胺HPLC-MS/MS检测[J]. 中国公共卫生, 2013, 29(5): 761-763. DOI: 10.11847/zgggws2013-29-05-51 |
| 作者姓名： | 张琦 马金波 |
| 作者单位： | 1.沈阳市疾病预防控制中心食品与公卫理化科,辽宁 110031;2.沈阳市环境监测中心站 |
| 摘 要： | 目的 建立可以消除水体基体干扰影响的水中痕量丙烯酰胺的高效液相色谱-串联质谱(HPLC-MS/MS)检测方法。方法 样品经滤膜过滤,加入同位素内标后,用ProElut LLE+液液萃取柱净化富集,采用HPLC-MS/MS进行检测。结果 HPLC-MS/MS检测方法在2~300 μg/L的范围内线性关系良好,相关系数r=0.999 7;对超纯水、水厂出厂水和地表水的加标回收率在90.0%~96.5%,RSD均<5%;方法的检出限为0.006 μg/L,定量限为0.02 μg/L。结论 HPLC-MS/MS检测方法可以消除由于水体基体干扰对丙烯酰胺测定的影响,适合生活饮用水和地表水中痕量丙烯酰胺的检测。 |
| 关 键 词： | 高效液相色谱-串联质谱(HPLC-MS/MS) 同位素内标 丙烯酰胺 |
| 收稿时间： | 2013-01-31 |
Elimination of matrix interference in determination of trace acrylamide in water with HPLC-MS/MS |
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| ZHANG Qi, MA Jin-bo. Elimination of matrix interference in determination of trace acrylamide in water with HPLC-MS/MS[J]. Chinese Journal of Public Health, 2013, 29(5): 761-763. DOI: 10.11847/zgggws2013-29-05-51 | |
| Authors: | ZHANG Qi MA Jin-bo |
| Affiliation: | 1.Department of Food Hygiene and Laboratory Detection for Public Health,Shenyang Municipal Center for Disease Control and Prevention,Shenyang,Liaoning Province 110031,China |
| Abstract: | Objective To establish a method to eliminate matrix interference in the determination of trace acrylamide in water with high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS) Methods After adding isotope,the samples were extracted and cleaned up and then acrylanide in the water samples was determined by HPLC-MS/MS.Results The calibration linear range of acrylamide was from 2 to 300 μg/L with a correlation coefficient of 0.9997.The average recoveries for different matrices were 90.0%-96.5% with the relative standard deviation(RSD) of less than<5%,detection limit of 0.006 μg/L,and limit of quantification of 0.02 μg/L.Conclusion The results show that the method is suitable for determination of trace acylamide in water. |
| Keywords: | HPLC-MS/MS isotope internal standard acrylamide |
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