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Er: YAG激光对2型糖尿病相关性牙周炎患者龈下菌群动态变化的研究
引用本文:郭斌 梁倩倩 吕岩 付敏 陈媛媛 袁一芳 李颖超. Er: YAG激光对2型糖尿病相关性牙周炎患者龈下菌群动态变化的研究[J]. 华西口腔医学杂志, 2014, 32(2): 171-176. DOI: 10.7518/hxkq.2014.02.015
作者姓名:郭斌 梁倩倩 吕岩 付敏 陈媛媛 袁一芳 李颖超
作者单位:1.中国人民解放军总医院口腔医学研究所,北京 100853;2.航空总医院口腔科,北京 100012; 3.口腔疾病研究国家重点实验室 华西口腔医院(四川大学),成都 610041
基金项目:国家自然科学基金资助项目(81271145);四川省科技支撑计划基金资助项目(2011FZO199)
摘    要:目的 动态观察2型糖尿病相关性牙周炎患者经Er: YAG激光治疗前后龈下菌群的变化,并与不伴全身疾病的慢性牙周炎龈下菌群进行比较。方法 收集11例2型糖尿病相关性牙周炎患者的13对患牙(26个位点)作为试验组,分别进行Er: YAG激光治疗和超声治疗,采集治疗前及治疗后1、3个月的龈下菌斑;同时收集11例牙周状况相近的不伴全身疾病的中重度慢性牙周炎患者13个位点的龈下菌斑作为对照组,分析试验组是否有菌群种类变化。提取龈下菌斑DNA,进行变性梯度凝胶电泳分离及条带回收测序。结果 试验组与对照组患牙龈下菌斑的优势致病菌存在差异,分别为中间普雷沃菌和福赛斯坦纳菌。激光组治疗前后龈下菌群构成也发生改变,治疗后1个月,有的条带表达减弱或消失,并有新条带出现;测序结果表明,新出现的条带为放线菌的一种,减弱、消失的为福赛斯坦纳菌。结论 Er: YAG激光治疗前后龈下菌群的构成发生变化,治疗后1个月是关键时期;治疗后3个月,激光治疗在阻止细菌再定植方面可能更具优势。

关 键 词:Er: YAG激光  2型糖尿病  慢性牙周炎  龈下菌群  变性梯度凝胶电泳  
收稿时间:2013-08-25
修稿时间:2014-01-20

Effect of Er: YAG laser on the dynamic changes in subgingival flora in type 2 diabetic patients with periodontitis
Guo Bin,Liang Qianqian,Lü Yan,Fu Min,Chen Yuanyuan,Yuan Yifang,Li Yingchao. Effect of Er: YAG laser on the dynamic changes in subgingival flora in type 2 diabetic patients with periodontitis[J]. West China journal of stomatology, 2014, 32(2): 171-176. DOI: 10.7518/hxkq.2014.02.015
Authors:Guo Bin  Liang Qianqian  Lü Yan  Fu Min  Chen Yuanyuan  Yuan Yifang  Li Yingchao
Affiliation:1. Research Institute of Oral Medi-cine, Chinese PLA General Hospital, Beijing 100853, China; 2. Dept. of Stomatology, Aviation General Hospital, Beijing 100012, China; 3. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
Abstract:Objective   To observe the changes in subgingival microflora before and after Er: YAG laser treatment on dia-betic patients with periodontitis, and to compare with the subgingival microflora of chronic periodontitis. Methods   Subgingival plaque of 13 pairs of teeth (26 sites) was selected from type 2 diabetic patients at pretreatment, one month post-treatment, and three months post-treatment. Subgingival plaque was also obtained from 11 cases of moderate to severe chronic periodon-titis with similar severity of periodontitis. The DNA of the subgingival plaque samples was extracted. Whole bacterial 16S rDNA gene fragments separated by denaturing gradient gel electrophoresis. Specific DNA bands were then chosen for retrieval and sequencing. Results   The gene sequencing results of the special DNA bands of subgingival plaque samples show that the pathogenic bacteria of both diabetic periodontitis and simple chronic periodontitis were Prevotella intermedia and Tannerella forsythia, respectively. The composition of the subgingival microflora before and after laser treatment changed. Some DNA bands, including that of Tannerella forsythia, disappeared or weakened one month after treatment. A new strip appeared, which belonged to Actinomyces sp. Conclusion   The profiles of the subgingival microflora changed after treatment, and one month was indicated as an important stage. Er: YAG laser may have an important function in delaying microflora recolonization.
Keywords:Er: YAG laser  type 2 diabetic')"   href="  #"  >type 2 diabetic  chronic periodontitis  subgingival plaque  ')"   href="  #"  >denaturing gradient gel electrophoresis
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