| 2型糖尿病发病进程中血清微小核糖核酸-103表达的临床意义 |
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| 引用本文： | 王奕,陈雪辉,陈红兵. 2型糖尿病发病进程中血清微小核糖核酸-103表达的临床意义[J]. 安徽医药, 2017, 21(10). DOI: 10.3969/j.issn.1009-6469.2017.10.023 |
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| 作者姓名： | 王奕 陈雪辉 陈红兵 |
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| 作者单位： | 南京医科大学附属儿童医院检验科,江苏南京,210008;南京市中医院检验科,江苏南京,210001 |
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| 摘 要： | 目的 探讨血清微小核糖核酸(miRNA)-103在2型糖尿病(T2DM)前期、新诊断的T2DM以及糖尿病并发症患者中的表达水平及临床意义.方法 选取70例研究对象,其中20例为糖耐量正常的个体(NGT组),18例为糖调节受损患者(prediabetes组),18例为新诊断的T2DM并无明显并发症患者(T2DM组),14例为T2DM并发症患者(T2DM伴并发症组).所有诊断均符合美国糖尿病学会(ADA)糖尿病诊断与分型标准中的诊断标准.应用荧光定量实时逆转录聚合酶链反应(PR-PCR)法评价各组受试者血清miRNA-103的表达水平.结果 Pre-diabetes组、T2DM组以及T2DM并发症组血清样本中miR-NA-103水平显著高于NGT组(P ＜0.05);T2DM组血清样本中miRNA-103水平显著高于pre-diabetes组(P＜0.05),而miR-NA-103在T2DM伴并发症组与T2DM组血清中的表达差异无统计学意义(P＞0.05).结论 血清miRNA-103有可能用于诊断及预测糖尿病的敏感的生物标志物,对于鉴别高风险患者具有至关重要的意义.
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| 关 键 词： | 糖调节受损 2型糖尿病 糖尿病并发症 微小核糖核酸-103 |
The clinical significance of expression level of serum microRNA-103 in the progression of type 2 diabetes mellitus |
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| WANG Yi,CHEN Xuehui,CHEN Hongbing. The clinical significance of expression level of serum microRNA-103 in the progression of type 2 diabetes mellitus[J]. Anhui Medical and Pharmaceutical Journal, 2017, 21(10). DOI: 10.3969/j.issn.1009-6469.2017.10.023 |
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| Authors: | WANG Yi CHEN Xuehui CHEN Hongbing |
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| Abstract: | Objective To investigate the expression levels of serum miRNA-103 in pre-diabetes,newly diagnosed type 2 diabetes mellitus without evident complications and type 2 diabetes mellitus with complications.Methods Selected 70 casesin this study,there were 20 healthy controls,18 patients with impaired glucose regulation (pre-diabetes),18 newly diagnosed type 2 diabetes mellitus without evident complications and 14 type 2 diabetes mellitus with complications.They were diagnosed according to American Diabetes Association diagnostic criteria.Serum miRNA-103 expression was assessed by quantitative polymerase chain reaction.Results We found a significant increase of serum miRNA-103 expression between pre-diabetes as well as diabetic patients when both compared with controls and between diabetic patients compared to pre-diabetes(P ＜ 0.05).There was no significant difference for serum miRNA-103 expression level between diabetic patients with complications and diabetic patients without evident complications (P ＞ 0.05).Conclusions Serum miRNA-103 expression canbe a good biomarker for diagnosis and prediction of pre-diabetes and type 2 diabetes mellitus as well as for better identification of a high risk form of diabetes. |
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| Keywords: | Impaired glucose regulation Type 2 diabetes mellitus Diabetic complications miRNA-103 |
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