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| 慢性丙型肝炎病毒感染者外周血淋巴细胞增殖反应 | |
| 引用本文： | 范学工,唐发清,欧志明,章锦香,刘国成,胡国龄. 慢性丙型肝炎病毒感染者外周血淋巴细胞增殖反应[J]. 世界华人消化杂志, 1999, 7(12): 1038-1040 |
| 作者姓名： | 范学工 唐发清 欧志明 章锦香 刘国成 胡国龄 |
| 作者单位： | 1. 湖南医科大学湘雅医院,湖南省长沙市,410008 2. 宁乡人民医院,湖南省宁乡县,420076 3. 宁乡中心血站,湖南省宁乡县,420076 |
| 基金项目： | 教育部高校优秀青年教师资助计划，卫生部优秀青年人才科研项目 |
| 摘 要： | 目的 观察慢性丙型肝炎患者外周血淋巴细胞对丙型肝炎病毒（ＨＣＶ） 抗原刺激的增殖反应．方法 外周血单个核细胞（ＰＢＭＣ） 与ＨＣＶ 抗原ｃ２２ 、ｃ３３ 、ｃ１００ － ３ 、ＮＳ５ 和植物血凝素（ＰＨＡ） 分别共同孵育，加入胸腺嘧啶核苷（３ ＨＴｄＲ） ，然后收集细胞于液闪仪测定每分钟脉冲数（ｃｐｍ） ．结果 根据对不同ＨＣＶ 抗原的淋巴细胞增殖反应发现，以ｃ２２免疫原性最强，ｃ１００ － ３ 次之：淋巴细胞激活与ＨＣＶ 基因型关系不大；健康对照和慢性乙型肝炎患者对各ＨＣＶ 抗原未能显示有效的淋巴细胞增殖反应；与健康对照比较，慢性丙型肝炎和乙型肝炎患者对ＰＨＡ 刺激的淋巴细胞增殖反应降低．结论 ＨＣＶ 抗原ｃ２２ 免疫原性最强，丙型肝炎患者对ＨＣＶ 抗原的淋巴细胞增殖反应系特异性；慢性丙型肝炎和乙型肝炎患者存在抑制的细胞免疫应答． |
| 关 键 词： | 肝硬化/病理学 肝/病理学 疾病模型,动物 大鼠,Wistar |
| 修稿时间： | 1999-07-14 |
Lymphoproliferative response to hepatitis C virus (HCV) antigens in patients with chronic HCV infection |
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| FAN Xue-Gong,TANG Fa-Qing,OU Zhi-Ming,ZHANG Jin-Xiang,LIU Guo-Cheng,HU Guo-Ling. Lymphoproliferative response to hepatitis C virus (HCV) antigens in patients with chronic HCV infection[J]. World Chinese Journal of Digestology, 1999, 7(12): 1038-1040 | |
| Authors: | FAN Xue-Gong TANG Fa-Qing OU Zhi-Ming ZHANG Jin-Xiang LIU Guo-Cheng HU Guo-Ling |
| Abstract: | AIM To observe the proliferative response of peripheral blood lymphocytes to hepatitis C virus (HCV) antigens in patients with chronic HCV infection. METHODS Peripheral lymphocytes were co cultured with HCV antigens c22, c33, c100-3 , NS5 and phytohemagglutinin (PHA), respectively. 3H TdR was then added and counts per minute (cpm) were measured. RESULTS Based on cpm, c22 was found to be the most immunogenic antigen, although all viral antigens were immunogenic for lymphocytes. Because lymphoproliferative responses to HCV antigens in healthy controls and patients with hepatitis B were not observed, lymphocyte response to HCV antigens in patients with HCV infection was considered to be specific. Furthermore, lymphocyte response to PHA was significantly decreased in patients with hepatitis C or hepatitis B compared to healthy controls ( P <0 001 , P <0 02 ). CONCLUSION HCV antigen c22 is the most immunogenic antigen for lymphocytes in the study. Suppressed cellular immune function is present in both patients with chronic hepatitis C and hepatitis B, which is partly responsible for chronicity of HCV infection in such patients. |
| Keywords: | hepatitis C virus lymphocytes antigens viral hepatitis C |
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