| 甲基硝基亚硝基胍诱导日本血吸虫成虫培养细胞增殖的研究 |
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| 引用本文： | 董惠芬, 蒋明森, 明珍平, 钟沁萍, 杨明义. 甲基硝基亚硝基胍诱导日本血吸虫成虫培养细胞增殖的研究[J]. 中国公共卫生, 2000, 16(10): 883-884. DOI: 10.11847/zgggws2000-16-10-08 |
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| 作者姓名： | 董惠芬 蒋明森 明珍平 钟沁萍 杨明义 |
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| 作者单位： | 1.湖北医科大学人体寄生虫学和血吸虫病研究室, 武汉430071 |
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| 基金项目： | 武汉市青年科技晨光计划湖北省基金和教育厅资助项目 |
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| 摘 要： | 将培养第5天的日本血吸虫成虫培养细胞在含甲基硝基亚硝基胍(MNNG)终浓度分别为0(对照)、1、2、3、4、6、9μg/ml的附加20%小牛血清及常量抗生素的RPMI1640培养基中分别处理24h、36h、48h;随后,换用含20%小牛血清及常量抗生素的RPMI1640培养基培养;3d后,改用含5%小牛血清及常量抗生素的RPMI1640培养基继续培养,每天在OlympusIM倒置显微镜下观察.用浓度为6μg/ml、9μg/ml的MNNG处理的日本血吸虫成虫培养细胞在处理后3天左右大片脱落,残留的少量细胞在换用5%小牛血清及常量抗生素的RPMI1640培养后逐渐死亡;用1、2、3、4μg/ml浓度MNNG处理后的日本血吸虫成虫培养细胞,生长良好,与对照组相比,培养细胞的体积变大,分裂细胞增多,尤以3μ/ml的MNNG处理48h的试验组最为明显,并观察到转化灶,但传代培养没有成功.结果表明在一定程度上,MNNG能够诱导日本血吸虫成虫培养细胞出现转化灶,并发生分裂、繁殖.
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| 关 键 词： | 日本血吸虫 培养细胞 MNNG 细胞增殖 |
| 收稿时间： | 1999-11-26 |
| 修稿时间： | 1999-11-26 |
The Influence of MNNG on the Proliferation of Cultured Cell from Adult Schistosoma Japonicum |
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| Dong Huifen, . The Influence of MNNG on the Proliferation of Cultured Cell from Adult Schistosoma Japonicum[J]. Chinese Journal of Public Health, 2000, 16(10): 883-884. DOI: 10.11847/zgggws2000-16-10-08 |
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| Authors: | Dong Huifen) |
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| Affiliation: | 1.Department of Medical Parasitology and Research Laboratory of Schistosomiasis, Hubei Medical University, Wuhan 430071 |
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| Abstract: | The function of MNNG on the proliferation of cultured cells from adult Schistosome were cultured in medium RPMI_1640 containing 20% calf serum and a moderate amount of antibiotics.Five days later,they were treated by MNNG with concentrations of 0,1,2,3,4,6 and 9μg/ml for 0,24,36 and 48 hours,respectively.Afterwards they were moved into the original medium.Three weeks later,the rate of calf serum in the medium was adjusted into to 5%.The proliferation and growth of the treated cells were observed by microscope everyday.Most of the cells treated by MMNG with concentrations of 6 and 9μg/ml fell off from the wall of culture flasks after 3 dyas,and the remained cells cultured in the medium containing 5% calf serum declined gradually.All the cells treated by MNNG of 1,2,3,and 4μg/ml grew well in the medium.The volume of the cells was larger and more cells in division were observed in the first groups than those in the control.especially in the cells treated by MNNG of the concentration of 3μg/ml for 48 hours.MNNG with the moderate concentration can induce the proliferation and division of cultured cells from adult Schistosoma japonicum. |
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| Keywords: | Schistosoma japonicum Cultured cells MNNG Cell proliferation |
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