Protection against anti-glomerular basement membrane (GBM)-mediated nephritis in C3- and C4-deficient mice |
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| Authors: | P J W VENABLES |
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| Affiliation: | Kennedy Institute of Rheumatology, London, UK |
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| Abstract: | Mice rendered completely deficient of the complement components C3 or C4 were used to determine the influence of complement activation in the heterologous phase of the anti-GBM disease model. In wild-type animals the disease is characterized by a neutrophil infiltrate, capillary thrombosis, proteinuria and C3 and C4 deposited within the glomerulus. The early infiltration of neutrophils into the glomeruli is greater in wild-type mice (2.8 ± 0.3) compared with C3-deficient (1.4 ± 0.2) and C4-deficient (1.2 ± 0.003) mice. Deficiency also protects against the subsequent development of proteinuria (2.99 ± 1.11 mg/24 h, 0.059 mg/24 h and 0.327 ± 0.14 mg/24 h in wild-type, C3-deficient and C4-deficient mice, respectively) and decreases glomerular capillary thrombosis in both C3- and C4-deficient mice. The degree of protection is greater in the C3-deficient than the C4-deficient animals, suggesting both classical and alternative pathway involvement. These studies support a critical role for complement in the development of anti-GBM disease. However, the protective effect of complement deficiency can be broken if the dose of nephritogenic antibody is increased. |
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| Keywords: | complement deficiency knockout mice anti-glomerular basement membrane disease glomerulonephritis |
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