| 日本血吸虫信号蛋白14-3-3的虫体免疫定位 |
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| 引用本文： | 刘庆中,沈继龙,汪学龙. 日本血吸虫信号蛋白14-3-3的虫体免疫定位[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(6): 330-332 |
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| 作者姓名： | 刘庆中 沈继龙 汪学龙 |
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| 作者单位： | 1. 温州医学院附属第一医院检验科,温州,325000
2. 安徽医科大学病原生物学教研室,安徽省基因研究重点实验室,合肥,230032 |
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| 基金项目： | 国家自然科学基金 (No:30 1 70 84)，安徽省自然科学基金 (No :0 0 4 4 5 4 7)资助项目~~ |
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| 摘 要： | 目的 研究抗重组日本血吸虫信号蛋白1433(rSj1433)单克隆抗体对天然Sjl433的结合活性,观察Sj1433在虫体内的定位。 方法 从阳性钉螺体内逸出尾蚴,感染家兔,分别于感染后15d和42d剖杀,静脉灌注法收集童虫和成虫制备冰冻切片。利用rSj1433单克隆抗体,间接免疫荧光法探讨信号蛋白1433虫体内的分布。 结果 免疫荧光染色结果显示,rSj1433单克隆抗体可特异性地结合天然Sj1433抗原表位,背景清晰,Sj1433广泛分布在雌、雄成虫的皮层、皮下层、肌层和实质层,前三种组织中特异性荧光呈线状分布,实质中特异性荧光弥散;童虫中Sj1433也广泛的分布在皮层、皮下层、肌层和实质层。 结论 免疫荧光染色成功地确定了Sj1433蛋白在成虫和15d童虫体内的分布
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| 关 键 词： | Sj14-3-3 克隆抗体 间接免疫荧光 定位 |
| 文章编号： | 1000-7423(2003)-06-0330-03 |
| 修稿时间： | 2003-03-31 |
Immunolocalization of the Signaling Protein l4-3-3 of Schistosoma japonicum |
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| LIU Qing-zhong,SHEN Ji-long,WANG Xue-ong. Immunolocalization of the Signaling Protein l4-3-3 of Schistosoma japonicum[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2003, 21(6): 330-332 |
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| Authors: | LIU Qing-zhong SHEN Ji-long WANG Xue-ong |
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| Affiliation: | Department of Pathobiology, Anhui Medical University, Hefei 230032. |
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| Abstract: | Objective To study the localization of the signaling protein 14-3-3 of Schistosoma japonicum (Sj14-3-3)in the parasite. Methods Cercariae were collected from the infected Oncomelania hupensis for the infection of rabbits. Fifteen-day-old schistosomula and adult worms obtained from infected rabbits 15 and 42 days post-infection were used for frozen sections and indirect immunofluorescence staining with monoclonal antibody to rSj14-3-3. Results The results showed that the Sj14-3-3 distributed mainly in the tegument, subtegument, muscle, and parenchyma of both adult worms and 15-day-old schistosomula. Conclusion The wide distribution and large sites of Sj14-3-3 in the parasite were clearly demonstrated, which established a significant clue for further studies of biologic actions and application of 14-3-3 protein. |
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| Keywords: | Schistosoma japonicum Sj14-3-3 monoclonal antibody indirect immunofluorescence localization |
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