| 虾青素脂质体对小鼠皮肤紫外线损伤干预机制的初步研究 |
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| 引用本文： | 李福民,刘 媛,廖金凤,等. 虾青素脂质体对小鼠皮肤紫外线损伤干预机制的初步研究[J]. 四川大学学报(医学版), 2018, 49(5): 712-715 |
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| 作者姓名： | 李福民 刘 媛 廖金凤 等 |
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| 作者单位： | 1.四川省医学科学院·四川省人民医院 皮肤科 |
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| 摘 要： | 目的 探讨虾青素脂质体对小鼠皮肤中波紫外线（UVB）损伤的干预作用及机制。方法 将40只C57BL/6J小鼠随机分为4组，即空白组（不行UVB照射，不用药）、模型组（UVB光损伤组，只照射不用药）、对照组（照射+虾青素）、实验组（照射+虾青素脂质体），每组10只。UVB照射（辐照强度为2 mW·cm2，辐照时间为60 s；前5日每日照射1次，后9日隔日照射1次，2周共照射10次）及药物干预（每次辐照前10 min用4 mL 0.2‰虾青素或4 mL 0.2‰虾青素脂质体涂抹于暴露皮肤）2周后，HE染色分别观察皮肤组织病理学改变，免疫组织化学染色观察皮肤 Ki-67抗原、8-羟基-2-脱氧鸟苷（8-OHdG）表达情况，试剂盒测定皮肤超氧化物岐化酶（SOD）活力和血清基质金属蛋白酶-13（MMP-13）质量浓度。结果 模型组和对照组的HE染色显示真皮变薄、真皮胶原纤维细长、排列疏松紊乱，与空白组比较，Ki-67、MMP-13及8-OHdG表达增加以及SOD活力降低，其差异均有统计学意义（P＜0.05）。实验组与模型组相比皮肤组织病理变化得到明显改善，Ki-67、MMP-13及8-OHdG表达减少及SOD活力升高，其差异具有统计学意义（P＜0.05）。结论 外用虾青素脂质体可改善小鼠皮肤光损伤引起的病理变化及减轻胶原损伤，其强抗氧化作用可能是干预机制之一。
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| 关 键 词： | 虾青素脂质体 光损伤 基质金属蛋白酶-13 超氧化物岐化酶 |
The Preliminary Study on Anti-photodamaged Effect of Astaxanthin Liposomes in Mice Skin |
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| LI Fu-min,LIU Yuan,LIAO Jin-feng,et al. The Preliminary Study on Anti-photodamaged Effect of Astaxanthin Liposomes in Mice Skin[J]. Journal of Sichuan University. Medical science edition, 2018, 49(5): 712-715 |
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| Authors: | LI Fu-min LIU Yuan LIAO Jin-feng et al |
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| Abstract: | Objective To study the protective effects of astaxanthin liposome (Asx-lipo) on photodamage by UVB in mice skin. Methods 40 C57BL/6J mice were randomly divided into four groups: The blank group (no irradiation, no drug use), model group (UVB light injury group, no drug use), control group (irradiation + astaxanthin), experimental group (irradiation + astaxanthin liposome), each group with 10 mice. Each group was given the corresponding light (the radiation intensity was 2 mW·cm2, the time of irradiation was 60 s, 1 times a day for the first 5 days, and 1 times every other day for the next 9 days, 10 times in a total of 2 weeks.) and drug intervention (topically treated with 4 mL 0.2‰ astaxanthin or 4 mL 0.2‰ Asx-lipo 10 min before the irradiation) for two weeks. After that, samples were examined by the following indicators: the histological changes of skin, Ki-67, 8-hydroxy-2′-deoxyguanosine(8-OHdG), superoxide dismutase(SOD) activities and serum matrix metalloproteinase-13 (MMP-13). Results HE staining the model group and the control group showed that the dermis became thin, the dermal collagen fibers were long and thin, and the arrangement was loose and disordered. Compared with the blank group, the expression of Ki-67, MMP-13 and 8-OHdG increased and SOD activity decreased, and the differences were statistically significant (P<0.05). Compared with the model group, the pathological changes of skin tissues in the experimental group were significantly improved, with decreased expressions of Ki-67, MMP-13 and 8-OHdG and increased SOD activity, and the differences were statistically significant (P<0.05).Conclusion The photodamage of mice skin can be improved by topical Asx-lipo. The mechanism may be related to the strong antioxidation of Asx-lipo. |
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| Keywords: | Astaxanthin liposome Photodamage MMP-13 SOD |
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