| 外源性胰岛素对电化学发光免疫分析法检测胰岛素的影响 |
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| 引用本文： | 张 玫,周 君,杨礼丹,等. 外源性胰岛素对电化学发光免疫分析法检测胰岛素的影响[J]. 四川大学学报(医学版), 2018, 49(6): 924-928 |
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| 作者姓名： | 张 玫 周 君 杨礼丹 等 |
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| 作者单位： | 1.四川大学华西医院 实验医学科 |
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| 摘 要： | 目的 探讨外源性胰岛素对电化学发光免疫分析法检测胰岛素的影响。方法 采用电化学发光免疫分析法测定血清胰岛素水平，根据美国临床实验室标准化协会标准文件EP7-A2的要求，采用配对差异实验分别评价8种外源性胰岛素对高、低浓度血清胰岛素测定的影响。如具有显著影响，用剂量效应实验评价确定外源性胰岛素浓度与干扰程度间的关系。 结果 甘舒霖N?、甘舒霖R?、优泌林R?、诺和灵R?、来得时?在干扰浓度 ≤ 250 μU /mL时，对高、低浓度胰岛素测定的影响均为线性正干扰；诺和平?在干扰物浓度 ≤ 250 μU /mL时，对高浓度胰岛素的影响为线性负干扰，对低浓度胰岛素无干扰，优泌乐?和诺和锐?对胰岛素检测无干扰。 结论 不同外源性胰岛素对电化学发光免疫分析法检测血清胰岛素的影响不同，临床上应注意由此导致的检测误差。
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| 关 键 词： | 干扰试验 外源性胰岛素 电化学发光免疫分析法 |
Interference of Exogenous Insulin on Insulin Detection Test by Electrochemiluminescence Immunoassay |
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| ZHANG Mei,ZHOU Jun,YANG Li-dan,et al. Interference of Exogenous Insulin on Insulin Detection Test by Electrochemiluminescence Immunoassay[J]. Journal of Sichuan University. Medical science edition, 2018, 49(6): 924-928 |
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| Authors: | ZHANG Mei ZHOU Jun YANG Li-dan et al |
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| Abstract: | Objective To explore the interference of exogenous insulin therapy on insulin detection test by electrochemical luminescence immunoassay (ECLIA). Methods Insulin level was determined by ECLIA. According to the requirements of EP7-A2 of American Society for Clinical Laboratory Standards Institute Standards, paired difference experiment was conducted to evaluate the interference of 8 kinds of exogenous insulin on insulin detection, dose effect experiment was conducted to determine the relationship between exogenous insulin concentration and interference degree. Results When the interfering substance concentrations were ≤250 μU/mL, Gansulin N?, Gansulin R?, Humulin R?，Novolin R? and Lantus? all showed linear positive interference, while Levemir? showed a linear negative interference in high concentrations insulin and non-interfering in low concentrations insulin, Humalog? and Novo Rapid? showed non-interference in insulin detection. Conclusion The use of different exogenous insulin may have different interference on insulin measurement, which need laboratorians and physicians notice to avoid misdiagnosis. |
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| Keywords: | Interference test Exogenous insulin Electrochemiluminescence immunoassay |
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