| 血清特异性免疫复合物在幽门螺杆菌感染诊断中的意义 |
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| 引用本文： | 王缚鲲,吴亚男,邹全明,冉向阳,时兰春,安黎云,王宪灵. 血清特异性免疫复合物在幽门螺杆菌感染诊断中的意义[J]. 实用医学杂志, 2006, 22(12): 1350-1352 |
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| 作者姓名： | 王缚鲲 吴亚男 邹全明 冉向阳 时兰春 安黎云 王宪灵 |
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| 作者单位： | 400038,重庆市,第三军医大学临床微生物学及免疫学教研室 |
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| 摘 要： | 目的:建立一种血清幽门螺杆菌(Helicobacterpylori,Hp)特异性免疫复合物的检测方法,并评价其在Hp感染诊断中的作用。方法:利用抗Hp单克隆抗体捕获Hp特异性免疫复合物,再用酶标羊抗人免疫球蛋白对其进行检测,并对其特异性和敏感性进行分析,同时与临床常用的血清特异性抗体检测、快速尿素酶试验、涂片镜检、14C尿素呼气试验和细菌培养5种方法进行了比较,并对药物根除效果进行了评价。结果:血清可溶性Hp抗原检测方法敏感性和特异性分别为86.0%和96.0%,此方法明显优于血清特异性抗体检测、快速尿素酶试验、涂片镜检和细菌培养,与14C尿素呼气试验差异无显著性,药物治疗前后血清Hp特异性免疫复合物含量差异无显著性。结论:血清Hp特异性免疫复合物检测方法是一种简便、高敏感性和特异性的检测Hp感染的方法,但不能用于Hp感染药物根除效果的评价。
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| 关 键 词： | 螺杆菌 幽门 免疫复合物 单克隆抗体 |
| 收稿时间： | 2006-02-07 |
| 修稿时间： | 2006-02-07 |
Diagnostic significance of serum specific immunocomplexes in Helicobacter pylori infection |
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| WANG Fu-kun,WU Ya-nan,ZOU Quan-ming,RAN Xiang-yang,SHI Lan-chun,AN Li-yun,WANG Xian-ling. Diagnostic significance of serum specific immunocomplexes in Helicobacter pylori infection[J]. The Journal of Practical Medicine, 2006, 22(12): 1350-1352 |
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| Authors: | WANG Fu-kun WU Ya-nan ZOU Quan-ming RAN Xiang-yang SHI Lan-chun AN Li-yun WANG Xian-ling |
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| Affiliation: | Department of Clinical Microbiology and Immunology, College of Laboratory Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Objective To develop a technique for detecting serum Helicobacter pylori (Hp) specific immunocomplexes (Hp- IC) and explore its diagnostic significance in Hp infection. Methods ELISA with monoclonal antibodies was applied to detect Hp- IC and its sensibility and specificity were analysed. It was then compared with Hp serum specific antibody assay, rapid urease test, smear for microscopy, 14C- urea breath test and bacterial culture. The eradicative efficacy of the anti- Hp agents was evaluated. Results The sensibility and specificity of the test were 86.0% and 96.0% respectively. This test was much better than Hp serum specific antibody assay, rapid urease test, smear for microscopy, bacterial culture, and had no significant difference compared with 14C- urea breath test. The content of soluble serum Hp antigen had not a markedly difference after the medical treatment. Conclusion This technique is simple and convenient to detect Hp infection with high sensibility and specificity, and can not be used for assessing the medical eradication of Hp. |
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| Keywords: | Helicobacter pylori Immunocomplex Monoclonal antibody |
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