| 宫颈癌组织 TMPRSS4、 LAD1 表达水平及与其患者预后的关系 |
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| 引用本文： | 金荣,曹霞,巢夏芬,吴雪琴,冯莉. 宫颈癌组织 TMPRSS4、 LAD1 表达水平及与其患者预后的关系[J]. 医学分子生物学杂志, 2022, 19(4): 320-325. DOI: 10.3870/j.issn.1672-8009.2022.04.009 |
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| 作者姓名： | 金荣 曹霞 巢夏芬 吴雪琴 冯莉 |
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| 作者单位： | 丹阳市人民医院 (南通大学附属丹阳医院) 妇产科 江苏省丹阳市, 212300 |
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| 摘 要： | 目的 探讨跨膜丝氨酸蛋白酶 4 ( transmembrane protease serine 4, TMPRSS4)、 线皮素-1 ( ladinin-1, LAD1) 在宫颈癌患者组织中的表达情况及与患者预后的关系。 方法 选取 2015 年 6 月 ~ 2018 年 6月在丹阳市人民医院手术治疗的宫颈癌患者 79 例为研究对象, 并在术中收集患者 79 例癌组织标本, 60 例癌旁组织标本, 采用免疫组织化学染色法观察宫颈癌组织中 TMPRSS4、 LAD1 蛋白表达情况, 实时荧光定量 PCR (qRT-PCR) 法检测组织中 TMPRSS4、 LAD1 mRNA 表达水平。 收集患者临床病理参数并对患者进行 3 年随访, 分析 TMPRSS4、 LAD1 表达水平与患者临床病理参数的关系及影响宫颈癌患者预后的因素。Pearson 法分析 TMPRSS4 与 LAD1 表达的相关性, 利用受试者工作特征曲线 (receiver operating characteristiccurve, ROC) 分析 TMPRSS4、 LAD1 水平对患者预后的预测价值。 结果 宫颈癌患者癌组织 TMPRSS4、LAD1 表达水平均高于癌旁组织 (P< 0. 05)。 宫颈癌组织中 TMPRSS4 和 LAD1 的表达水平呈明显正相关 (r =0. 249, P< 0. 05)。 宫颈癌组织中 TMPRSS4 与 LAD1 蛋白表达与患者 FIGO 分期和肿瘤分化程度有关 (P<0. 05), FIGO 分期、 肿瘤分化程度、 TMPRSS4、 LAD1 蛋白表达水平是影响患者预后的因素 (P< 0. 05)。 预后存活患者癌组织中 TMPRSS4 和 LAD1 水平均显著低于预后死亡患者 (P< 0. 05)。 ROC 结果显示, TMPRSS4 和 LAD1 水平评估患者预后的曲线下面积分别为 0. 756、 0. 909, 对应的敏感度分别为 91. 11 % 、52. 94 % , 特异度分别为 85. 29 % 、 85. 29 % ; 二者联合评估患者预后的曲线下面积为 0. 952, 敏感度为88. 89 % , 特异度为 88. 24 % 。 结论 TMPRSS4、 LAD1 在宫颈癌患者组织中均高表达, 二者表达呈明显正相关, 可应用于评估患者预后。
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| 关 键 词： | 宫颈癌 跨膜丝氨酸蛋白酶 4 线皮素-1 预后 |
Expression of TMPRSS4 and LAD1 in Cervical Cancer Tissues andTheir Relationship with the Prognosis in Patients |
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| JIN Rong,CAO Xia,CHAO Xiafen,WU Xueqin,FENG Li. Expression of TMPRSS4 and LAD1 in Cervical Cancer Tissues andTheir Relationship with the Prognosis in Patients[J]. Journal of Medical Molecular Biology, 2022, 19(4): 320-325. DOI: 10.3870/j.issn.1672-8009.2022.04.009 |
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| Authors: | JIN Rong CAO Xia CHAO Xiafen WU Xueqin FENG Li |
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| Affiliation: | Department of Obstetrics and Gynecology, Danyang People’s Hospital (Danyang Hospital Affiliatedto Nantong University), Danyang, Jiangsu, 212300, China |
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| Abstract: | Objective To investigate the expression of transmembrane protease serine 4 (TMPRSS4) and LAD1 in cervical cancer tissues and their relationship with the prognosis of cervicalcancer patients. Methods A total of 79 patients with cervical cancer who underwent surgical treatment in Danyang People’s Hospital from June 2015 to June 2018 were selected as the research objects. The 79 cancerous tissue specimens and 60 para-cancerous tissue specimens were collected fromcervical cancer patients during the operation. The immunohistochemical staining method and realtime fluorescent quantitative PCR (qRT-PCR) method were used to detect the protein and mRNAexpression levels of TMPRSS4 and LAD1 in tissues. The clinical pathological parameters of the patients were collected and the patients were followed up for 3 years. The relationship between the expression levels of TMPRSS4 and LAD1 and the clinical pathological parameters of patients, as wellas the factors that affect the prognosis of patients with cervical cancer were analyzed. The correlationbetween TMPRSS4 and LAD1 expression was analyzed with Pearson method. The predictive value ofTMPRSS4 and LAD1 levels on the prognosis of patients was analyzed with the receiver operatingcharacteristic curve (ROC). Results The expression levels of TMPRSS4 and LAD1 in cervicalcancer tissues were higher than those in adjacent tissues (P < 0. 05). The expression levels of TMPRSS4 and LAD1 in cervical cancer tissues were significantly positively correlated (r = 0. 249, P<0. 05). The expression levels of TMPRSS4 and LAD1 proteins in cervical cancer tissue were relatedto the FIGO stage and tumor differentiation degree (P < 0. 05). FIGO staging, tumor differentiation, TMPRSS4 and LAD1 protein expression levels were the factors affecting the prognosis of patients (P< 0. 05). The expression levels of TMPRSS4 and LAD1 in the cancer tissues of patients insurvival group were significantly lower than those in death group ( P < 0. 05 ). The ROC resultsshowed that the areas under the curve (AUCs) for TMPRSS4 and LAD1 to assess the prognosis ofpatients were 0. 756 and 0. 909, respectively, the corresponding sensitivities of the assessment were91. 11 % and 52. 94 % respectively, and the specificities were 85. 29 % and 85. 29 % respectively. The AUC for the combination of using TMPRSS4 and LAD1 to assess the prognosis of patients was0. 952, the sensitivity of the assessment was 88. 89 % , and the specificity was 88. 24 % . Conclusion TMPRSS4 and LAD1 are both highly expressed in cervical cancer tissues. The expression levels of TMPRSS4 and LAD1 are significantly positively correlated, and they can be used for the prognosis of cervical cancer patients. |
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| Keywords: | cervical cancer transmembrane protease serine 4 ladinin-1 prognosis |
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